Epidermal growth factor enhances transcription of human arachidonate 12-lipoxygenase in A431 cells
| Autor: | Ching Jiunn Chen, Ben Kuen Chen, Toshiya Arakawa, Tanihiro Yoshimoto, Shozo Yamamoto, Wen Chang Chang, Yi Wen Liu |
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| Rok vydání: | 1997 |
| Předmět: |
Transcription
Genetic Biophysics Biology Arachidonate 12-Lipoxygenase Transfection Biochemistry Endocrinology Cytosol Epidermal growth factor Transcription (biology) Genes Reporter Microsomes Tumor Cells Cultured Humans Luciferase RNA Messenger Luciferases Promoter Regions Genetic Nucleic Acid Synthesis Inhibitors Messenger RNA Epidermal Growth Factor Promoter beta-Galactosidase Molecular biology Enzyme Activation Epidermoid carcinoma Gene Expression Regulation Carcinoma Squamous Cell Dactinomycin A431 cells hormones hormone substitutes and hormone antagonists Dichlororibofuranosylbenzimidazole |
| Zdroj: | Biochimica et biophysica acta. 1344(1) |
| ISSN: | 0006-3002 |
| Popis: | Epidermal growth factor (EGF), determined by immunoprecipitation and Western blot analysis, increased both enzyme activity and protein level of 12-lipoxygenase in the solubilized microsomes of human epidermoid carcinoma A431 cells, respectively. The EGF-induced expression of 12-lipoxygenase mRNA was inhibited by transcription inhibitors such as actinomycin D and 5,6-dichlorobenzimidazole riboside. Promoters of different lengths for human 12-lipoxygenase gene were used to prepare the luciferase fusion vectors. These construct plasmids were transiently transfected into A431 cells, and the induction of luciferase expression by EGF was examined. A 4- to 6-fold increase in luciferase reporter activity stimulated by EGF for 18 h treatment was observed in plasmids with the 5′-flanking region length of −951 bp and that of −224 bp upstream from translation starting site. The time-dependent induction of luciferase activity by EGF paralleled the EGF-induced enzyme activity and expression of 12-lipoxygenase protein. Taken together, the results of this study indicate that EGF enhanced the transcription of the human 12-lipoxygenase gene, resulting in an increase in the amount and activity of 12-lipoxygenase. |
| Databáze: | OpenAIRE |
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