Analysis of the indanomycin biosynthetic gene cluster from Streptomyces antibioticus NRRL 8167
| Autor: | Chaoxuan Li, Wendy L. Kelly, Kathryn E. Roege |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Mutagenesis (molecular biology technique) Biochemistry Cyclase Thioesterase Transferases Polyketide synthase Gene cluster Pyrroles Amino Acid Sequence Gene Silencing Molecular Biology Gene Pyrans Sequence Deletion biology Chemistry Organic Chemistry Streptomyces antibioticus Deletion Mutagenesis Anti-Bacterial Agents Open reading frame Mutagenesis Multigene Family biology.protein Molecular Medicine Macrolides Polyketide Synthases |
| Zdroj: | Chembiochem : a European journal of chemical biology. 10(6) |
| ISSN: | 1439-7633 |
| Popis: | Metabolites that harbor a core indane scaffold are found to have diverse biological properties. Indanomycin and related pyrroloketoindanes are ionophores and have demonstrated antiparasitic, insecticidal, and antibacterial activities. To understand the biochemical mechanisms guiding formation of the central indane ring, the biosynthetic gene cluster for indanomycin was identified from Streptomyces antibioticus NRRL 8167 and sequenced to approximately 80 kb; this revealed five genes encoding subunits of a polyketide synthase (PKS) and 18 other open reading frames. The involvement of this cluster in indanomycin biosynthesis was confirmed by deletion mutagenesis. The indanomycin PKS lacks the expected thioesterase at the carboxy terminus of the final module, and instead appears to house an incomplete module containing an unusual cyclase domain. These findings now enable additional detailed genetic and biochemical studies of the mechanisms guiding the generation of pyrroloketoindanes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text