Growth and lipid production of Umbelopsis isabellina on a solid substrate - Mechanistic modeling and validation
| Autor: | Johannes Tramper, Petra Meeuwse, Sebastiaan Haemers, Anne J. Klok, Arjen Rinzema |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Bio Process Engineering
aspergillus-oryzae state fermentation system chemistry.chemical_element Bioengineering Raw material Biology biodiesel production Applied Microbiology and Biotechnology Biochemistry Oxygen Chemical kinetics Exponential growth gamma-linolenic acid oleaginous fungi glucose VLAG chemostat model Biodiesel chemistry diffusion-coefficients Yield (chemistry) Biodiesel production Fermentation accumulation oxygen |
| Zdroj: | Process Biochemistry 47 (2012) 8 Process Biochemistry, 47(8), 1228-1242 |
| ISSN: | 1359-5113 |
| Popis: | Microbial lipids are an interesting feedstock for biodiesel. Their production from agricultural waste streams by fungi cultivated in solid-state fermentation may be attractive, but the yield of this process is still quite low. In this article, a mechanistic model is presented that describes growth, lipid production and lipid turnover in a culture of Umbelopsis isabellina on κ-carrageenan plates containing the monomers glucose and alanine as C-source and N-source, respectively, and improves the understanding of the complex solid-state system. The model includes reaction kinetics and diffusion of glucose, alanine and oxygen. It is validated empirically and describes the different phases of the culture very well: exponential growth, linear growth because of oxygen limitation, accumulation of lipids and carbohydrates after local N-depletion and turnover of lipids after local C-depletion. Extending the model with an unidentified extracellular product improved the fit of the model to the data. The model shows that oxygen limitation is extremely important in solid-state cultures using monomers, and explains the difference in production rate with submerged cultures. However, the results also show that the specific lipid production rate in solid-state cultures is much lower than in submerged cultures, which results in a low lipid yield. |
| Databáze: | OpenAIRE |
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