Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry
| Autor: | Bommana Naresh Kumar, Vasireddy Veera Vara Prasad, K. Seshaiah, Padala Venkateswarlu |
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| Rok vydání: | 2010 |
| Předmět: |
Pharmacology
Chromatography Reverse-Phase Chromatography Chemistry Extraction (chemistry) Reproducibility of Results Pharmaceutical Science General Medicine Tandem mass spectrometry Mass spectrometry Adrenergic beta-1 Receptor Antagonists High-performance liquid chromatography Electron capture detector Tandem Mass Spectrometry Humans Sample preparation Solid phase extraction Gas chromatography Chromatography High Pressure Liquid Metoprolol |
| Zdroj: | Acta Pharmaceutica. 60 |
| ISSN: | 1846-9558 1330-0075 |
| DOI: | 10.2478/v10007-010-0012-0 |
| Popis: | Metoprolol, 1-isopropylamino-3-[4-(2-methoxy-ethyl)-phenoxy]-2-propanol, is a b1-selective aryloxy propanolamine used in the treatment of cardiovascular disorders such as hypertension, arrhythmia and heart failure (1). The drug is a lipophilic adrenoreceptor antagonist (b-blocker) with a short half-life. Various methods reported for the determination of metoprolol and its metabolites in human plasma include gas chromatography (GC) equipped with an electron capture detector (ECD) (2), nitrogen selective detector (NSD) (3), mass spectrometry (MS) detector (4, 5) and high performance liquid chromatography equipped with a ultra violet detector (6, 7), fluorescence detector (8–12), MS detector (13–15). Sample preparation for the extraction of metoprolol from human plasma is based on the solid phase extraction (4) and liquid-liquid extraction using dichloro methane (2, 8, 10, 12), n-butyl chloride (11) and mixture of dichloromethane and diethyl ether (2, 8). |
| Databáze: | OpenAIRE |
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