Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
| Autor: | Estefania Vangelie Ramos Campos, Tais Germano da Costa, Daniele Ribeiro de Araujo, Patrícia L. F. Proença, Sarah Hedtrich, Renata de Lima, Leonardo Fernandes Fraceto |
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| Přispěvatelé: | Federal University of Abc, Universidade Estadual Paulista (UNESP), University of Sorocaba (UNISO), The University of British Columbia |
| Rok vydání: | 2021 |
| Předmět: |
Budesonide
budesonide atopic dermatitis Polymers and Plastics Chemistry Process Chemistry and Technology Organic Chemistry Nanoparticle Atopic dermatitis Pharmacology medicine.disease polymeric nanoparticles thermoresponsive hydrogels Self-healing hydrogels Percutaneous absorption medicine topical release medicine.drug |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 2637-6105 |
| DOI: | 10.1021/acsapm.1c00021 |
| Popis: | Made available in DSpace on 2022-05-01T08:45:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-10 Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense itching and recurrent eczematous lesions. Topical corticosteroids are the first-line treatment to control moderate-to-severe AD; however, prolonged application of corticosteroids is required, which can result in dermal atrophy as a side effect. Drug-delivery systems can provide more effective and targeted therapy strategies. In this study, budesonide (BUD) was encapsulated into chitosan (CS)-coated PLGA nanoparticles, which were further incorporated into poloxamer hydrogels to improve the anti-inflammatory activity and decrease adverse effects. The nanoparticles were prepared by the emulsification-solvent evaporation technique, and their physicochemical characteristics were evaluated. Rheological properties of the hydrogels, such as viscosity and sol-gel transition temperature, were evaluated with and without nanoparticles. In vitro release kinetics and ex vivo drug absorption studies were performed using Franz diffusion cells. The nanoparticles showed a mean diameter of 324 ± 4 nm, positive ζ potential (20 mV) due to CS coating, and high encapsulation efficiency (>90%). The nanoparticles did not show cytotoxic effects in primary human fibroblasts and keratinocytes; however, all formulations induced the generation of reactive oxygen species. Both nanoparticles and hydrogels were able to change the release kinetics of BUD when compared to the nonencapsulated compound. Nanoparticles were not able to surmount the stratum corneum of excised human skin, but the nanoencapsulation facilitated the skin absorption of BUD. The hydrogels containing nanoparticles or not showed non-Newtonian and pseudoplastic behavior. The nanoformulations seem to be a good candidate to deliver glucocorticoids in the skin of AD patients. Human and Natural Sciences Center Federal University of Abc, Sao Paulo Drugs and Bioactives Delivery Systems Research Group - Sislibio Federal University of Abc, Sao Paulo Department of Environmental Engineering Institute of Science and Technology Sao Paulo State University - Unesp, Sao Paulo Laboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials University of Sorocaba (UNISO), Sao Paulo Faculty of Pharmaceutical Sciences The University of British Columbia Department of Environmental Engineering Institute of Science and Technology Sao Paulo State University - Unesp, Sao Paulo |
| Databáze: | OpenAIRE |
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