Activity of Sanguinarine against Candida albicans Biofilms
| Autor: | Yan Wang, Zhuo Er Zhang, Zheng Wang, Yuan Ying Jiang, Gan Hai Hu, Ri Li Zhang, Shuang Bi, Zheng Xu, Hua Zhong, Dan Dan Hu, Juan Su |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antifungal Agents Hypha 030106 microbiology Hyphae Down-Regulation Endogeny Microbial Sensitivity Tests Umbilical vein Microbiology 03 medical and health sciences chemistry.chemical_compound Candida albicans Cell Adhesion Cyclic AMP Human Umbilical Vein Endothelial Cells Humans Pharmacology (medical) Sanguinarine Cells Cultured Benzophenanthridines Pharmacology biology Chemistry Biofilm Isoquinolines biology.organism_classification Corpus albicans Infectious Diseases Gene Expression Regulation Biofilms cAMP-dependent pathway Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 61 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.02259-16 |
| Popis: | Candida albicans biofilms show resistance to many clinical antifungal agents and play a considerable contributing role in the process of C. albicans infections. New antifungal agents against C. albicans biofilms are sorely needed. The aim of this study was to evaluate sanguinarine (SAN) for its activity against Candida albicans biofilms and explore the underlying mechanism. The MIC 50 of SAN was 3.2 μg/ml, while ≥0.8 μg/ml of SAN could suppress C. albicans biofilms. Further study revealed that ≥0.8 μg/ml of SAN could decrease cellular surface hydrophobicity (CSH) and inhibited hypha formation. Real-time reverse transcription-PCR (RT-PCR) results indicated that the exposure of C. albicans to SAN suppressed the expression of some adhesion- and hypha-specific/essential genes related to the cyclic AMP (cAMP) pathway, including ALS3 , HWP1 , ECE1 , HGC1 , and CYR1 . Consistently, the endogenous cAMP level of C. albicans was downregulated after SAN treatment, and the addition of cAMP rescued the SAN-induced filamentation defect. In addition, SAN showed relatively low toxicity to human umbilical vein endothelial cells, the 50% inhibitory concentration (IC 50 ) being 7.8 μg/ml. Collectively, the results show that SAN exhibits strong activity against C. albicans biofilms, and the activity was associated with its inhibitory effect on adhesion and hypha formation due to cAMP pathway suppression. |
| Databáze: | OpenAIRE |
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