Pathogenesis and expression profile of selected immune genes to experimental Edwardsiella tarda infection in iridescent shark Pangasianodon hypophthalmus
| Autor: | Pathakota Gireesh-Babu, Jitendra Kumar Sundaray, Aparna Chaudhari, Bhawesh T. Sawant, Nilambari Pawar, Rajdeep Dutta, Farhana Hoque, Pranali Pitale |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Immune gene expression
Spleen Aquatic Science Pangasianodon lcsh:Aquaculture. Fisheries. Angling Microbiology Edwardsiellosis Pathogenesis 03 medical and health sciences medicine Mortality Necrobiotic changes 030304 developmental biology lcsh:SH1-691 0303 health sciences Kidney Innate immune system biology Edwardsiella tarda Acute-phase protein 04 agricultural and veterinary sciences biology.organism_classification Tissue specificity medicine.anatomical_structure 040102 fisheries 0401 agriculture forestry and fisheries Animal Science and Zoology Catfish |
| Zdroj: | Aquaculture Reports, Vol 17, Iss, Pp 100371-(2020) |
| ISSN: | 2352-5134 |
| Popis: | Pathogenesis and immune gene expression of the iridescent shark catfish Pangasianodon hypophthalmus to experimental Edwardsiella tarda infection was studied. Healthy Pangasianodon were inoculated intramuscularly with a sub-lethal dose of 1.77 × 10⁷ cells/fish of E. tarda to induce edwardsiellosis. Petechial haemorrhages, inflammation at the site of injection, vertical hanging from the water surface, swollen abdomen and anorexia were the common signs observed in challenged catfish fingerlings. The morbid catfish also exhibited typical signs of septicaemia with haemorrhages on ventral parts of the body and fin bases and congested vent prior to death. The mortality was observed 48 h post injection. The average percent morality was 42 % in fish exposed to 1.77 × 10⁷ cells of E. tarda, whereas, only6.6 % mortality was observed in placebo control (catfish received 0.1 mL of 0.85 % sterile physiological saline) and no mortality in negative control group (catfish received no injection) over an observational period of 20 days. The kidney and liver of moribund fish demonstrated different necrobiotic changes. In kidney, cellular and cloudy swelling, degeneration of renal tubules, necrosis of glomerulus, dilation of Bowman’s space, hypertrophy, fibrosis of renal tubules and increased melano-macrophage reaction were frequently observed. In liver, disorganized hepatocytes, blood congestion and nuclear pyknosis were evident. To ascertain the host response to E. tarda infection, mRNA levels of three different classes of innate immune components viz. cytokine interleukin-1β (IL-1β), acute phase protein transferrrin and complement C3 were assessed by quantitative real-time PCR. Significant up-regulation (P |
| Databáze: | OpenAIRE |
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