Size and complexity of polyadenylated RNAs induced in tobacco infected with sonchus yellow net virus

Autor:	M.A. Rezaian, L.A. Heaton, Andrew O. Jackson, Joel J. Milner, Karl Pedersen
Rok vydání:	1983
Předmět:	Genetics Polyadenylation biology Nicotiana tabacum RNA Rhabdoviridae biology.organism_classification Genome Virology Virus law.invention Viral replication law Recombinant DNA
Zdroj:	Virology. 131:221-229
ISSN:	0042-6822
DOI:	10.1016/0042-6822(83)90547-0
Popis:	Hybridization of 32P-labeled sonchus yellow net virus (SYNV) RNA to polyadenylated (poly A+) RNA from infected tobacco reveals the presence of four electrophoretically distinct components. These components probably represent five discrete RNA species complementary to SYNV RNA (scRNAs). The scRNAs are smaller than the 13,000 nucleotide (NT) SYNV genome and range in size from 1200 to 6600 NT. Individual recombinant DNA clones derived from SYNV RNA hybridize to at least three and probably four of the scRNAs. These results suggest that each of the scRNAs contains unique sequences with a combined size representing more than 90% of the viral genome. Therefore, the size range and sequence complexity of the scRNAs are as expected for messenger RNAs encoding the four major SYNV polypeptides and the minor "L-protein."
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f949c1a70cac4a2a396e6d9eaa7ece0 https://doi.org/10.1016/0042-6822(83)90547-0 Zobrazit plný text záznamu