A single nucleotide polymorphism in EZH2 predicts overall survival rate in patients with cholangiocarcinoma
| Autor: | R. Tartarini, Elisa Giovannetti, Antonello A. Romani, Maurizio Cantore, Elisa Paolicchi, M. Orlandi, Francesco Crea, Paola Pacetti, Romano Danesi, Godefridus J. Peters, Andrea Mambrini |
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| Přispěvatelé: | Medical oncology laboratory, CCA - Innovative therapy |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Oncology
Cancer Research medicine.medical_specialty Oncogene EZH2 ECX regimen Single-nucleotide polymorphism Articles Single nucleotide polymorphisms Biology Cholangiocarcinoma Prognosis Bioinformatics Molecular medicine polymorphism SNP genotyping Malignant transformation Internal medicine Genotype medicine SNP polycomb |
| Zdroj: | Oncology Letters, 6(5), 1487-1491. Spandidos Publications Oncology Letters Paolicchi, E, Pacetti, P, Giovannetti, E, Mambrini, A, Orlandi, M, Crea, F, Romani, A A, Tartarini, R, Danesi, R, Peters, G J & Cantore, M 2013, ' A single nucleotide polymorphism in EZH2 predicts overall survival rate in patients with cholangiocarcinoma ', Oncology Letters, vol. 6, no. 5, pp. 1487-1491 . https://doi.org/10.3892/ol.2013.1559 |
| ISSN: | 1792-1074 |
| DOI: | 10.3892/ol.2013.1559 |
| Popis: | Cholangiocarcinoma (CCA) is a deadly disease arising from the malignant transformation of cholangiocytes. Enhancer of zeste homolog 2 (EZH2) is overexpressed in poorly differenti- ated CCA. Functional single nucleotide polymorphisms (SNPs) in this gene may affect the role of EZH2 in cholangiocarcino- genesis and chemoresistance. The aim of the current study was to evaluate the correlation between EZH2 SNPs and clinical outcome. Using PROMO3.0, GeneCard and MicroSNiper, 4 EZH2 SNPs with functional relevance in CCA were selected in silico. These SNPs were studied in genomic DNA extracted from the blood samples of 75 patients with advanced CCA, who were treated with epirubicin-cisplatin-xeloda (ECX regimen). SNP genotyping was performed with specific PCR assays. The rs887569 TT genotype was correlated with a significantly longer overall survival (OS; TT vs. CT-CC, P=0.026). Moreover, the TT genotype revealed a trend toward a significant association with a reduced risk of mortality (HR, 0.59; 95% CI, 0.33-1.05; P=0.075), by multivariate analysis. These results support future studies on the role of rs887569 EZH2 SNP as a possible predic- tive marker of OS in advanced CCA patients. |
| Databáze: | OpenAIRE |
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