Chemokine Receptor 7 Is Essential for Coxiella burnetii Whole-Cell Vaccine-Induced Cellular Immunity but Dispensable for Vaccine-Mediated Protective Immunity
| Autor: | Robert Faris, Phillip L. Felgner, James E. Samuel, Adam Vigil, Erin J. van Schaik, Anthony E. Gregory, Chen Chen, Laura R. Hendrix |
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| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Cellular immunity 030106 microbiology chemical and pharmacologic phenomena Immunoglobulin G Mice Major Articles and Brief Reports 03 medical and health sciences Immune system Antigen Immunity Animals Humans Immunology and Allergy Antigens Bacterial Immunity Cellular biology Vaccination Dendritic Cells Coxiella burnetii biology.organism_classification Antibodies Bacterial 030104 developmental biology Infectious Diseases Antibody Formation Bacterial Vaccines Immunology biology.protein bacteria Female Receptors Chemokine Antibody Q Fever |
| Zdroj: | J Infect Dis |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiz146 |
| Popis: | Background Protective immunity against Coxiella burnetii infection is conferred by vaccination with virulent (PI-WCV), but not avirulent (PII-WCV) whole-cell inactivated bacterium. The only well-characterized antigenic difference between virulent and avirulent C. burnetii is they have smooth and rough lipopolysaccharide (LPS), respectively. Methods Mice were vaccinated with PI-WCV and PII-WCV. Humoral and cellular responses were evaluated using protein chip microarrays and ELISpots, respectively. Dendritic cell (DC) maturation after stimulation with PI-WVC and PII-WVC was evaluated using flow cytometry. Vaccine-challenge studies were performed to validate the importance of the receptor CCR7. Results Other than specific antibody response to PI-LPS, similar antibody profiles were observed but IgG titers were significantly higher after vaccination with PI-WCV. Furthermore, higher frequency of antigen-specific CD4+ T cells was detected in mice immunized with PI-WCV. PI-WCV–stimulated DCs displayed significantly higher levels of CCR7 and migratory ability to secondary lymphoid organs. Challenge-protection studies in wild-type and CCR7-deficient mice confirmed that CCR7 is critical for PI-WCV–induced cellular immunity. Conclusions PI-WVC stimulates protective immunity to C. burnetii in mice through stimulation of migratory behavior in DCs for protective cellular immunity. Additionally, the humoral immune response to LPS is an important component of protective immunity. |
| Databáze: | OpenAIRE |
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