Ferret brain possesses young interneuron collections equivalent to human postnatal migratory streams
| Autor: | Patrick S. McQuillen, David H. Rowitch, Sasha Mikhailova, Justin K. Ellis, Khalida Sabeur, Manideep Chavali, Shawn F. Sorrells, Sandra Chang |
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| Přispěvatelé: | Rowitch, David H [0000-0002-0079-0060], Apollo - University of Cambridge Repository |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Doublecortin Domain Proteins RRID: AB_1640532 RRID:AB_1586992 postnatal migration Rostral migratory stream RRID:AB_2195374 RRID:AB_305869 RRID:AB_2298772 posterior migratory stream 0302 clinical medicine Gyrus RRID:AB_882455 doublecortin Cell Movement Cortex (anatomy) Prefrontal cortex ferret Research Articles dorsal migratory stream RRID:AB_10615497 prefrontal cortex Caspase 3 General Neuroscience Brain Human brain medial migratory stream White Matter medicine.anatomical_structure Microtubule-Associated Proteins Research Article Doublecortin Protein Interneuron Biology cortical interneurons 03 medical and health sciences RRID:AB_2194626 Interneurons secretagogin medicine Animals Humans RRID:AB_10000320 RRID:AB_477010 Neuropeptides Ferrets RRID: AB839504 Doublecortin RRID:AB_561007 030104 developmental biology nervous system biology.protein RRID:AB_1079874 Occipital lobe Neuroscience 030217 neurology & neurosurgery Secretagogins RRID:AB_2069869 |
| Zdroj: | The Journal of Comparative Neurology |
| DOI: | 10.17863/cam.39919 |
| Popis: | The human early postnatal brain contains late migratory streams of immature interneurons that are directed to cortex and other focal brain regions. However, such migration is not observed in rodent brain, and whether other small animal models capture this aspect of human brain development is unclear. Here, we investigated whether the gyrencephalic ferret cortex possesses human‐equivalent postnatal streams of doublecortin positive (DCX+) young neurons. We mapped DCX+ cells in the brains of ferrets at P20 (analogous to human term gestation), P40, P65, and P90. In addition to the rostral migratory stream, we identified three populations of young neurons with migratory morphology at P20 oriented toward: (a) prefrontal cortex, (b) dorsal posterior sigmoid gyrus, and (c) occipital lobe. These three neuronal collections were all present at P20 and became extinguished by P90 (equivalent to human postnatal age 2 years). DCX+ cells in such collections all expressed GAD67, identifying them as interneurons, and they variously expressed the subtype markers SP8 and secretagogin (SCGN). SCGN+ interneurons appeared in thick sections to be oriented from white matter toward multiple cortical regions, and persistent SCGN‐expressing cells were observed in cortex. These findings indicate that ferret is a suitable animal model to study the human‐relevant process of late postnatal cortical interneuron integration into multiple regions of cortex. The human early postnatal brain contains late migratory streams of immature interneurons directed toward prefrontal cortex and other brain regions. We investigated the early postnatal ferret cortex and identified three populations of young neurons with migratory morphology. These three populations expressed GAD67, identifying them as interneurons, and they variously expressed the subtype markers SP8 and secretagogin (SCGN). SCGN+ interneurons appeared to be oriented from white matter toward multiple cortical regions, and persistent SCGN‐expressing cells were observed in cortex. These findings indicate that ferret is a suitable animal model to study the human‐relevant process of late postnatal cortical interneuron integration into multiple regions of cortex. |
| Databáze: | OpenAIRE |
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