An exploration of the methods to determine the protein‐specific synthesis and breakdown rates in vivo in humans
| Autor: | Simon Doessing, Grith Højfeldt, Kasper Dideriksen, Marcus Moberg, Rie Harboe Nielsen, Søren Reitelseder, Gerrit van Hall, Eva Blomstrand, Rasmus Bechshoeft, Lars Holm |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Fysiologi Skeletal Muscle Physiology Muscle Proteins Connective tissue Phenylalanine 030204 cardiovascular system & hematology lcsh:Physiology Tendons 03 medical and health sciences 0302 clinical medicine In vivo Physiology (medical) stable isotope medicine Humans deuterated alanine Muscle Skeletal Essential amino acid Original Research Aged chemistry.chemical_classification fractional breakdown rate Alanine deuterated water Nitrogen Isotopes lcsh:QP1-981 Chemistry protein turnover Protein turnover Middle Aged Deuterium Amino acid Protein catabolism medicine.anatomical_structure Biochemistry Amino acid recycling fractional synthesis rate Cellular Physiology Myofibril Protein Processing Post-Translational 030217 neurology & neurosurgery |
| Zdroj: | Physiological Reports, Vol 7, Iss 17, Pp n/a-n/a (2019) Holm, L, Dideriksen, K, Nielsen, R H, Doessing, S, Bechshoeft, R L, Højfeldt, G, Moberg, M, Blomstrand, E, Reitelseder, S & van Hall, G 2019, ' An exploration of the methods to determine the protein-specific synthesis and breakdown rates in vivo in humans ', Physiological Reports, vol. 7, no. 17, e14143 . https://doi.org/10.14814/phy2.14143 Physiological Reports |
| Popis: | The present study explores the methods to determine human in vivo protein‐specific myofibrillar and collagenous connective tissue protein fractional synthesis and breakdown rates. We found that in human myofibrillar proteins, the protein‐bound tracer disappearance method to determine the protein fractional breakdown rate (FBR) (via 2H2O ingestion, endogenous labeling of 2H‐alanine that is incorporated into proteins, and FBR quantified by its disappearance from these proteins) has a comparable intrasubject reproducibility (range: 0.09–53.5%) as the established direct‐essential amino acid, here L‐ring‐13C6‐phenylalanine, incorporation method to determine the muscle protein fractional synthesis rate (FSR) (range: 2.8–56.2%). Further, the determination of the protein breakdown in a protein structure with complex post‐translational processing and maturation, exemplified by human tendon tissue, was not achieved in this experimentation, but more investigation is encouraged to reveal the possibility. Finally, we found that muscle protein FBR measured with an essential amino acid tracer prelabeling is inappropriate presumably because of significant and prolonged intracellular recycling, which also may become a significant limitation for determination of the myofibrillar FSR when repeated infusion trials are completed in the same participants. |
| Databáze: | OpenAIRE |
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