The extracellular matrix molecules versican and hyaluronan in urethral and vaginal tissues in stress urinary incontinence
| Autor: | Stephen P. Evanko, Una J. Lee, Chong H Choe, Bhavin N Patel, Eugene W Lee, Marika Bogdani, Ingrid A. Harten, Thomas N. Wight |
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| Rok vydání: | 2021 |
| Předmět: |
Urology
Urinary Incontinence Stress 030232 urology & nephrology Connective tissue Inflammation Pathogenesis Andrology Extracellular matrix Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Urethra medicine Animals Humans Hyaluronic Acid Messenger RNA 030219 obstetrics & reproductive medicine biology business.industry Middle Aged Extracellular Matrix Rats Disease Models Animal medicine.anatomical_structure Vagina biology.protein Immunohistochemistry Versican Female Neurology (clinical) medicine.symptom business Elastin |
| Zdroj: | Neurourology and urodynamicsREFERENCES. 40(3) |
| ISSN: | 1520-6777 |
| Popis: | Purpose Abnormal extracellular matrix (ECM) changes are correlated with stress urinary incontinence (SUI). The ECM components versican (Vcan) and hyaluronan (HA) play key roles in regulating tissue inflammation and maintaining connective tissue homeostasis. We analyzed the localization and expression of these ECM components in urethral and vaginal tissues from a rat model of urinary incontinence and from human clinical specimens. Methods Nulliparous rats underwent vaginal distension (VD), a rodent model of SUI, or a sham procedure. Tissues were harvested from six rats per group at days 1, 4, and 21 for immunohistochemistry and RNA expression analysis of ECM components. Periurethral vaginal samples from female patients with SUI were also examined. Results High-intensity staining for Vcan was observed 1 day after procedure in both control and VD animals. This level of abundance persisted at day 4 in VD compared to control, with concurrent reduced messenger RNA (mRNA) expression of the Vcan-degrading enzymes ADAMTS5 and ADAMTS9 and reduced staining for the Vcan cleavage epitope DPEAAE. Abundance of HA was not different between VD and control, however mRNA expression of the HA synthase Has2 was significantly reduced in VD tissues at day 4. Abundant Vcan staining was observed in 60% of SUI patient samples, which was strongest in regions of disrupted elastin. Conclusion Reduction of Vcan-degrading enzymes and HA synthases at day 4 postsurgery indicates a potential delay in ECM turnover associated with SUI. Abundant Vcan is associated with inflammation and elastin fiber network disruption, warranting further investigation to determine its role in SUI pathogenesis. |
| Databáze: | OpenAIRE |
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