Congenital tremor in piglets: Is bovine viral diarrhea virus an etiological cause?

Autor: Samir Issa Samara, Luís Guilherme de Oliveira, Andressa de Souza-Pollo, Felipe dos Santos Gomes, Marina Lopes Mechler, Karla Alvarenga Nascimento, Felipe Ferreira Barbosa Pires, Edviges Maristela Pituco
Přispěvatelé: Universidade Estadual Paulista (Unesp), Biological Institute of São Paulo
Rok vydání: 2018
Předmět:
Myoclonus
0301 basic medicine
Cerebellar hypoplasia
Swine
040301 veterinary sciences
Developmental Disabilities
Placenta
animal diseases
Physiology
Biology
Antibodies
Viral
Nervous System Malformations
Real-Time Polymerase Chain Reaction
Microbiology
Virus
0403 veterinary science
03 medical and health sciences
Fetus
Pregnancy
Cerebellum
Tremor
medicine
Animals
Diarrhea Virus 2
Bovine Viral
Pregnancy Complications
Infectious
Experimental infection
Swine Diseases
General Veterinary
Pestivirus
Antibody titer
Brain
04 agricultural and veterinary sciences
General Medicine
medicine.disease
biology.organism_classification
Animals
Suckling
Piglets
030104 developmental biology
RNA
Viral
Gestation
Bovine Virus Diarrhea-Mucosal Disease
Cattle
Female
Cerebellar hypoplasia (non-human)
Intrauterine inoculation
Blood sampling
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0378-1135
Popis: Made available in DSpace on 2018-12-11T17:37:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Congenital tremor in pigs involves several etiologies, including pestivirus, which may cause neurological injuries in different animal species. To evaluate whether bovine viral diarrhea virus (BVDV), an important pestivirus, is one of the etiological agents of congenital tremor in swine, gilts and the fetuses were challenged at 45 days of gestation with BVDV-2. Four pregnant gilts were inoculated oronasally, four gilts underwent fetal intrauterine inoculation, and two gilts constituted the control group. Antibody titers were determined by virus neutralization (VN), and viral RNA was detected by RT-PCR. Blood samples were collected from all gilts and piglets born to obtain whole blood and serum for analysis. One third of the neonates were euthanized at three days old, and samples of the encephalon, brain stem and spinal cord were collected for anatomopathological evaluation and viral RNA detection. The piglets that remained alive were clinically evaluated every day, and blood sampling was performed regularly for 35 days. The piglets from gilts in both inoculation treatment groups showed no clinical neurological signs and were born with no viral RNA in their blood and organs. Piglets born from oronasally inoculated gilts did not present antibodies against BVDV-2 at birth, although they were acquired by passive maternal transfer. In contrast, intrauterine-inoculated piglets were born with high antibody titers (80 to 640) against the agent, which remained high until the end of the experimental period. Microscopically, no noticeable changes were observed. Macroscopically, 29.5% of the total piglets euthanized, from both inoculation groups, were born with a low cerebellar:brain ratio. Nevertheless, some piglets had a high cerebellar:brain ratio, indicating the need for standardizing this value. Thus, it was concluded that BVDV is not an etiological agent for congenital swine tremor. São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), Via de Acesso Prof. Paulo Donato Castellane s/n Biological Institute of São Paulo, Av. Conselheiro Rodrigues Alves, 1252 - Vila Mariana São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences (FCAV), Via de Acesso Prof. Paulo Donato Castellane s/n FAPESP: 2016/21421-2 CNPq: 409435/2016-3
Databáze: OpenAIRE
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