An in vitro study on the antioxidant capacity of usnic acid on human erythrocytes and molecular models of its membrane
| Autor: | Małgorzata Jemioła-Rzemińska, Fernando Villena, Juan Pablo Staforelli, Mario Suwalsky, C. Astudillo, Kazimierz Strzałka, María José Gallardo |
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| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular antioxidant Antioxidant Erythrocytes Hypochlorous acid medicine.medical_treatment erythrocyte membrane Echinocyte Lipid Bilayers Biophysics Phospholipid Biochemistry Hemolysis Antioxidants Cell membrane Phospholipid bilayer chemistry.chemical_compound X-Ray Diffraction medicine Humans Lipid bilayer Cell Shape Cells Cultured Benzofurans Microscopy Chromatography Dose-Response Relationship Drug Molecular Structure Phosphatidylethanolamines usnic acid Erythrocyte Membrane Usnic acid Cell Biology Hypochlorous Acid medicine.anatomical_structure Membrane chemistry phospholipid bilayer Microscopy Electron Scanning Dimyristoylphosphatidylcholine |
| Zdroj: | Biochimica et biophysica acta. 1848(11 Pt) |
| ISSN: | 0006-3002 |
| Popis: | Usnic acid (UA) has been associated with chronic diseases through its antioxidant action. Its main target is the cell membrane; however, its effect on that of human erythrocytes has been scarcely investigated. To gain insight into the molecular mechanisms of the interaction between UA and cell membranes human erythrocytes and molecular models of its membrane have been utilized. Dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE) were chosen as representative of phospholipid classes located in the outer and inner monolayers of the erythrocyte membrane, respectively. Results by X-ray diffraction showed that UA produced structural perturbations on DMPC and DMPE bilayers. DSC studies have indicated that thermotropic behavior of DMPE was most strongly distorted by UA than DMPC, whereas the latter is mainly affected on the pretransition. Scanning electron (SEM) and defocusing microscopy (DM) showed that UA induced alterations to erythrocytes from the normal discoid shape to echinocytes. These results imply that UA molecules were located in the outer monolayer of the erythrocyte membrane. Results of its antioxidant properties showed that UA neutralized the oxidative capacity of HClO on DMPC and DMPE bilayers; SEM, DM and hemolysis assays demonstrated the protective effect of UA against the deleterious oxidant effects of HClO upon human erythrocytes. |
| Databáze: | OpenAIRE |
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