Survival of human prostate carcinoma, benign hyperplastic prostate tissues, and IL-2-activated lymphocytes in scid mice
| Autor: | L D Schultz, David M. Lubaroff, W G Beamer, Michael B. Cohen |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
endocrine system Pathology medicine.medical_specialty Neoplasms Hormone-Dependent Cell Survival Urology Prostatic Hyperplasia Spleen Mice SCID Biology Adenocarcinoma Mice Lymphocytes Tumor-Infiltrating Prostate medicine Tumor Cells Cultured Animals Humans Testosterone Severe combined immunodeficiency Cancer Prostatic Neoplasms Histology Prostate-Specific Antigen medicine.disease Flow Cytometry Lymphocyte Subsets Prostate-specific antigen Disease Models Animal medicine.anatomical_structure Oncology Dihydrotestosterone Interleukin-2 Neoplasm Transplantation medicine.drug |
| Zdroj: | The Prostate. 27(1) |
| ISSN: | 0270-4137 |
| Popis: | Mice, homozygous for the mutation severe combined immunodeficiency (scid) and also segregating for the mutation hypogonadal (hpg), were tested for their potential use as an in vivo model system for studying the growth of human prostate cancer and benign hyperplastic prostate tissue grafts. Fresh human prostate cancer or benign hyperplastic prostate tissue was implanted subcutaneously into androgen-replete C.B. 17 scid/scid males, and into androgen-deficient hpg/hpg scid/scid or androgen-replete +/? scid scid males. The tissue grafts grew in both androgen-replete and androgen-deficient host mice. When dihydrotestosterone (DHT) was administered at tissue grafting, both the incidence and size of the tissue grafts increased. Histology of tissue from tumors in the androgen-deficient hpg/hpg scid/scid host showed either undifferentiated tumors or adenocarcinomas with few glandular structures. These data suggest the androgen deficient environment selected for growth of androgen-independent tumor tissue. Finally, when interleukin-2 (IL-2)-activated tumor-infiltrating lymphocytes were injected into scid/scid hosts, the cells were found to survive and could be identified in the spleen of the recipient mice. These results indicate that growth of human prostate tissues and IL-2-activated lymphocytes in scid/scid mice is a viable model system for in vivo studies of prostatic disease. |
| Databáze: | OpenAIRE |
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