UVB-induced formation of (6-4) photoproducts in the rat corneal epithelium
| Autor: | Henrik S. Huitfeldt, Erling Haaskjold, Svein Estil, W. M. Olsen |
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| Rok vydání: | 1997 |
| Předmět: |
DNA damage
Ultraviolet Rays Mitosis Epithelium Cornea chemistry.chemical_compound In vivo Minimal erythema dose medicine Animals Rats Wistar Corneal epithelium biology business.industry Dose-Response Relationship Radiation DNA Fluoresceins Molecular biology Immunohistochemistry Rats Ophthalmology medicine.anatomical_structure chemistry Microscopy Fluorescence Pyrimidine Dimers biological sciences biology.protein Optoelectronics Fluorescein sense organs Antibody business DNA Damage |
| Zdroj: | Acta ophthalmologica Scandinavica. 75(2) |
| ISSN: | 1395-3907 |
| Popis: | The induction of DNA photoproducts in rat corneal epithelium was studied after in vivo exposure to different doses of ultraviolet B light at 297 nm. Affinity-purified antibodies with a major specificity against UV-induced (6–4) photoproducts were used. The results indicate a dose dependent formation of (6–4) photoproducts. Even a minimal erythema dose (25 mJ/cm2) produced (6–4) photoproducts, demonstrating that DNA damage occurs in corneal tissue following exposure to biologically relevant doses of UVB light. |
| Databáze: | OpenAIRE |
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