The Vacuole and Mitochondria Patch (vCLAMP) Protein Vam6 is Crucial for Autophagy in Candida albicans
| Autor: | Li Yang, Congcong Ma, Dixiong Yu, Xiaolong Mao, Mingchun Li, Qilin Yu, Tianyu Ma, Jiazhen Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Veterinary (miscellaneous) ATG8 030106 microbiology Mutant Vacuole Mitochondrion Applied Microbiology and Biotechnology Microbiology Fungal Proteins 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Mitophagy Candida albicans Autophagy biology Chemistry biology.organism_classification Cell biology Mitochondria Cytoplasm Vacuoles Agronomy and Crop Science |
| Zdroj: | Mycopathologia. 186(4) |
| ISSN: | 1573-0832 |
| Popis: | Vacuole and mitochondria patches (vCLAMPs) are involved in the stress resistance of yeast, but their exact role in autophagy remains so far unclear. This study, for the first time, investigated the role of the vCLAMP core protein Vam6 in autophagy of Candida albicans. The experiments demonstrated that the deletion of VAM6 led to a growth defect under nitrogen starvation. Also, western blotting revealed that the vam6Δ/Δ mutant attenuated degradation of Atg8 (an autophagy indicator), Lap41 (an indicator of the cytoplasm to vacuole targeting pathway), and Csp37 (a mitophagy indicator). Moreover, the activity of carboxypeptidase Y and the levels of the vacuolar phospholipase Atg15 were significantly decreased in the mutant, which confirmed the defect of autophagy caused by deletion of VAM6. Overall, these results revealed that Vam6 is essential in maintaining the autophagic process under nitrogen starvation, and this provided new insights into the correlation between vCLAMPs and autophagy. |
| Databáze: | OpenAIRE |
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