Mechanical wounding induces a nitrosative stress by down-regulation of GSNO reductase and an increase in S-nitrosothiols in sunflower (Helianthus annuus) seedlings
| Autor: | Francisco Luque, Juan C. Begara-Morales, Francisco J. Corpas, Alfonso Carreras, Beatriz Sánchez-Calvo, Mounira Chaki, Raquel Valderrama, Marina Leterrier, Juan B. Barroso, Ana Fernández-Ocaña, José Rafael Pedrajas, María V. Gómez-Rodríguez |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
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Physiology Plant Science protein tyrosine nitration Nitric Oxide Nitrate Reductase peroxynitrite Nitric oxide Superoxide dismutase S-Nitrosoglutathione chemistry.chemical_compound Gene Expression Regulation Plant Stress Physiological Homeostasis S-nitrosoglutathione Reactive nitrogen species Nitrites chemistry.chemical_classification Reactive oxygen species nitrotyrosine Nitrates S-Nitrosothiols biology Nitrotyrosine Hydrogen Peroxide Abiotic stress Research Papers Aldehyde Oxidoreductases Reactive Nitrogen Species Hypocotyl Nitric oxide synthase Cold Temperature Light intensity chemistry Biochemistry mechanical wounding biology.protein Helianthus Stress Mechanical |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Nitric oxide (NO) and related molecules such as peroxynitrite, S-nitrosoglutathione (GSNO), and nitrotyrosine, among others, are involved in physiological processes as well in the mechanisms of response to stress conditions. In sunflower seedlings exposed to five different adverse environmental conditions (low temperature, mechanical wounding, high light intensity, continuous light, and continuous darkness), key components of the metabolism of reactive nitrogen species (RNS) and reactive oxygen species (ROS), including the enzyme activities L-arginine-dependent nitric oxide synthase (NOS), S-nitrosogluthathione reductase (GSNOR), nitrate reductase (NR), catalase, and superoxide dismutase, the content of lipid hydroperoxide, hydrogen peroxide, S-nitrosothiols (SNOs), the cellular level of NO, GSNO, and GSNOR, and protein tyrosine nitration [nitrotyrosine (NO(2)-Tyr)] were analysed. Among the stress conditions studied, mechanical wounding was the only one that caused a down-regulation of NOS and GSNOR activities, which in turn provoked an accumulation of SNOs. The analyses of the cellular content of NO, GSNO, GSNOR, and NO(2)-Tyr by confocal laser scanning microscopy confirmed these biochemical data. Therefore, it is proposed that mechanical wounding triggers the accumulation of SNOs, specifically GSNO, due to a down-regulation of GSNOR activity, while NO(2)-Tyr increases. Consequently a process of nitrosative stress is induced in sunflower seedlings and SNOs constitute a new wound signal in plants. |
| Databáze: | OpenAIRE |
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