Specific Dietary Oligosaccharides Increase Th1 Responses in a Mouse Respiratory Syncytial Virus Infection Model
| Autor: | Belinda Van't Land, Marcel A. Schijf, Debby Kruijsen, Grada M. van Bleek, Johan Garssen, Jacqueline Bastiaans, Frank E. J. Coenjaerts |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
CD4-Positive T-Lymphocytes
T cell Immunology Oligosaccharides Respiratory Syncytial Virus Infections CD8-Positive T-Lymphocytes Biology Microbiology Virus Cytomegalovirus Vaccines Interferon-gamma Mice Immune system Virology medicine Animals Immunologic Factors Interferon gamma Lung Innate immune system Body Weight Th1 Cells respiratory system Diet Respiratory Syncytial Viruses Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Insect Science biology.protein Pathogenesis and Immunity Female Antibody Breast feeding CD8 medicine.drug |
| Zdroj: | Journal of Virology. 86:11472-11482 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Breast feeding reduces the risk of developing severe respiratory syncytial virus (RSV) infections in infants. In addition to maternal antibodies, other immune-modulating factors in human milk contribute to this protection. Specific dietary prebiotic oligosaccharides, similar to oligosaccharides present in human milk, were evaluated in a C57BL/6 mouse RSV infection model. During primary RSV infection, increased numbers of RSV-specific CD4 + T cells producing gamma interferon (IFN-γ) were found in the lungs at days 8 to 10 postinfection in mice receiving diet containing short-chain galactooligosacharides, long-chain fructooligosaccharides, and pectin-derived acidic oligosaccharides (termed scGOS/lcFOS/pAOS). In a Th2-skewed formalin-inactivated (FI)-RSV vaccination model, the prebiotic diet reduced RSV-specific Th2 cytokine (interleukin-4 [IL-4], IL-5, and IL-13)-producing CD4 + T cells in the lung and the magnitude of airway eosinophilia at day 4 and 6 after infection. This was accompanied by a decreased influx of inflammatory dendritic cells (CD11b + /CD11c + ) and increased numbers of IFN-γ-producing CD4 + and CD8 + T cells at day 8 after viral challenge. These findings suggest that specific dietary oligosaccharides can influence trafficking and/or effector functions of innate immune, CD4 + , and CD8 + T cell subsets in the lungs of RSV-infected mice. In our models, scGOS/lcFOS/pAOS had no effect on weight but increased viral clearance in FI-RSV-vaccinated mice 8 days after infection. The increased systemic Th1 responses potentiated by scGOS/lcFOS/pAOS might contribute to an accelerated Th1/Th2 shift of the neonatal immune system, which might favor protective immunity against viral infections with a high attack rate in early infancy, such as RSV. |
| Databáze: | OpenAIRE |
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