Flammulin: A novel ribosome-inactivating protein from fruiting bodies of the winter mushroom Flammulina velutipes
| Autor: | T B Ng, H X Wang |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Molecular Sequence Data
Biochemistry Fungal Proteins Reticulocyte Protein biosynthesis medicine Amino Acid Sequence Ribonuclease Molecular Biology Peptide sequence Plant Proteins Fungal protein Mushroom Cell-Free System biology Ribosome-inactivating protein Osmolar Concentration food and beverages Cell Biology Hydrogen-Ion Concentration biology.organism_classification Molecular Weight medicine.anatomical_structure Protein Biosynthesis biology.protein Agaricales Ribosomes Flammulina |
| Zdroj: | Biochemistry and Cell Biology. 78:699-702 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/o00-087 |
| Popis: | A protein with a molecular weight of 40 kDa, capable of inhibiting cell-free translation in a rabbit reticulocyte lysate system with an IC50 of 0.25 nM, was isolated from fruiting bodies of the mushroom Flammulina velutipes. The protein, designated flammulin, was devoid of ribonuclease activity. Flammulin was unadsorbed on DEAE-cellulose at neutral pH and low ionic strength and adsorbed on CM-Sepharose and Affi-gel blue gel under similar conditions. Its N-terminal sequence demonstrates sites of similarity to those of plant ribosome-inactivating proteins (RIPs).Key words: mushroom, ribosome-inactivating protein, fruiting body. |
| Databáze: | OpenAIRE |
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