Gastrointestinal Stromal Tumors and Leiomyomas in the Dog: A Histopathologic, Immunohistochemical, and Molecular Genetic Study of 50 Cases
| Autor: | J. Lasota, D. Frost, M. Miettinen |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty 040301 veterinary sciences Molecular Sequence Data Abdominal cavity Polymerase Chain Reaction Metastasis 0403 veterinary science 03 medical and health sciences Dogs medicine Animals Large intestine Amino Acid Sequence Dog Diseases Esophagus Gastrointestinal Neoplasms Base Sequence Leiomyoma General Veterinary biology GiST CD117 Stomach DNA Neoplasm 04 agricultural and veterinary sciences medicine.disease Immunohistochemistry digestive system diseases Proto-Oncogene Proteins c-kit 030104 developmental biology medicine.anatomical_structure Mutation biology.protein Female Stromal Cells Sequence Alignment |
| Zdroj: | Veterinary Pathology. 40:42-54 |
| ISSN: | 1544-2217 0300-9858 |
| Popis: | Fifty canine gastrointestinal (GI) mesenchymal tumors were examined to determine the occurrence of leiomyomas (LM) and GI stromal tumors and to compare their clinicopathologic features. Twenty-one tumors (42%) were histologically reclassified as gastrointestinal stromal tumors (GISTs) and 29 tumors (58%) as LMs on the basis of their histologic similarity with homologous human tumors. The GISTs occurred equally in males and females, with a mean age of 11 years (range 5–14 years). Five GISTs (24%) were associated with clinical signs and six (29%) had metastasis in liver or abdominal cavity. The GISTs occurred in large intestine (10, 48%), small bowel (six, 29%), stomach (four, 19%), and mesentery of small intestine (one, 5%). Histologically, they were highly cellular spindle, or less commonly epithelioid tumors with mitotic rates ranging from 0 to 19 per 10 HPF. Eleven tumors (52%) were positive for CD117 (KIT); seven (33%) were positive for smooth muscle actin but none for desmin and S-100 protein. Sequences of KIT exon 11, often mutated in human GISTs, were evaluated from four GISTs. Deletion of Try556-Lys557 coexisting with duplication of Gln555 in one case of GIST and T to C transition resulting in substitution of Pro for Leu575 in another were identified. The LMs occurred predominantly in males (82%) with a mean age of 11 years (range 8–17 years). Nine tumors (31%) had associated clinical signs. They occurred in the stomach (22, 76%), esophagus (four, 14%), and intestines (three, 10%); all were paucicellular, had no mitoses, and were composed of mature smooth muscle cells. Twenty-eight (97%) were positive for smooth muscle actin and 18(62%) for desmin but none for CD117 and S-100. Both GISTs and true LMs occur in the GI tract of dogs. Both tumors have distinctive pathologic features. |
| Databáze: | OpenAIRE |
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