Automated identification and characterisation of microbial populations using flow cytometry: the AIMS project

Autor: Linda K. Medlin, Lynne Boddy, R. R. Jonker, Laura García, Glen A. Tarran, René Groben, M. F. Wilkins, Laura Zabala
Jazyk: angličtina
Rok vydání: 2000
Předmět:
Zdroj: Scientia Marina, Vol 64, Iss 2, Pp 225-234 (2000)
ResearcherID
Scientia Marina; Vol. 64 No. 2 (2000); 225-234
Scientia Marina; Vol. 64 Núm. 2 (2000); 225-234
Scientia Marina
Consejo Superior de Investigaciones Científicas (CSIC)
Scientia Marina; Vol 64, No 2 (2000); 225-234
ISSN: 1886-8134
0214-8358
DOI: 10.3989/scimar.2000.64n2
Popis: The AIMS (Automatic Identification and characterisation of Microbial populationS) project is developing and integrating flow cytometric technology for the identification of microbial cell populations and the determination of their cellular characteristics. This involves applying neural network approaches and molecular probes to the identification of cell populations, and deriving and verifying algorithms for assessing the chemical, optical and morphometric characteristics of these populations. The products of AIMS will be calibrated data, protocols, algorithms and software designed to turn flow cytometric observations into a data matrix of the abundance and cellular characteristics of identifiable populations. This paper describes the general approach of the AIMS project, with details on the application of artificial neural nets and rRNA oligonucleotide probes.
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Databáze: OpenAIRE