Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway

Autor: Zejing Wang, Hao Lu, Tao Tang, Lei Liu, Bohan Pan, Jiqiu Chen, Dasheng Cheng, Xiaoxiao Cai, Yu Sun, Feng Zhu, Shihui Zhu
Rok vydání: 2022
Předmět:
Zdroj: Cell Proliferation. 55
ISSN: 1365-2184
0960-7722
Popis: To determine the therapeutic effect of tetrahedral framework nucleic acids (tFNAs) on diabetic wound healing and the underlying mechanism.The tFNAs were characterized by polyacrylamide gel electrophoresis (PAGE), atomic force microscopy (AFM), transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential assays. Cell Counting Kit-8 (CCK-8) and migration assays were performed to evaluate the effects of tFNAs on cellular proliferation and migration. Quantitative polymerase chain reaction (Q-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the effect of tFNAs on growth factors. The function and role of tFNAs in diabetic wound healing were investigated using diabetic wound models, histological analyses and western blotting.Cellular proliferation and migration were enhanced after treatment with tFNAs in a high-glucose environment. The expression of growth factors was also facilitated by tFNAs in vitro. During in vivo experiments, tFNAs accelerated the healing process in diabetic wounds and promoted the regeneration of the epidermis, capillaries and collagen. Moreover, tFNAs increased the secretion of growth factors and activated the Wnt pathway in diabetic wounds.This study indicates that tFNAs can accelerate diabetic wound healing and have potential for the treatment of diabetic wounds.
Databáze: OpenAIRE
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