LC–MS analysis and cytoprotective effect against the mercurium and aluminium toxicity by bioactive products of Psidium brownianum Mart. ex DC
| Autor: | Ana Raquel Pereira da Silva, José Galberto Martins da Costa, Celestina E. Sobral-Souza, Henrique Douglas Melo Coutinho, Nadghia Figueiredo Leite, Irwin Rose Alencar de Menezes, Larissa Araújo Rolim, Francisco Afrânio Cunha |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
Antioxidant Iron Health Toxicology and Mutagenesis medicine.medical_treatment Flavonoid 0211 other engineering and technologies Germination 02 engineering and technology 010501 environmental sciences 01 natural sciences Antioxidants Mass Spectrometry chemistry.chemical_compound Candida albicans Escherichia coli medicine Aluminum Chloride Environmental Chemistry Gallic acid Waste Management and Disposal Chelating Agents 0105 earth and related environmental sciences Flavonoids chemistry.chemical_classification 021110 strategic defence & security studies Psidium Chromatography Plant Extracts Quinic acid Lettuce Pollution chemistry Cytoprotection Mercuric Chloride Myricetin Quercetin Allelopathy Chromatography Liquid Ellagic acid |
| Zdroj: | Journal of Hazardous Materials. 370:54-62 |
| ISSN: | 0304-3894 |
| DOI: | 10.1016/j.jhazmat.2018.03.039 |
| Popis: | This study aimed to verify the chelating, antioxidant and cytoprotective activities of Psidium brownianum Mart. Ex DC against mercury and aluminum. The ethanolic extract, as well as the tannic and flavonoid fractions, were prepared and subjected to liquid chromatography-mass spectrometry analysis. Ferric ion reduction and antioxidant activity measurement using the FRAP method were performed with P. brownianum. After determining the sub-allelopathic doses, germination tests using Lactuca sativa (lettuce) seeds were performed. The main compounds identified in the extract and fractions were: quercetin and its derivatives; myricetin and its derivatives; gallic acid; ellagic acid; quinic acid and gallocatechin. The Minimum Inhibitory Concentration (MIC) for all samples were ≥ 1024 μg/mL. The flavonoid fraction in association with mercury chloride demonstrated cytoprotection (p 0.001). The sub-allelopathic concentration used was 64 μg/mL. The extract and fractions were cytoprotective for radicles and caulicles when assayed in association with mercury and against aluminum for radicles. This suggests that the P. brownianum extract and its fractions present cytoprotective activity, possibly related to the antioxidant effect of secondary metabolites, especially flavonoids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect