Publisher Correction: Ex vivo real-time monitoring of volatile metabolites resulting from nasal odorant metabolism
| Autor: | Elisabeth Guichard, Philippe Faure, Aline Robert-Hazotte, Etienne Sémon, Rachel Schoumacker, Jean-Luc Le Quéré, Jean-Marie Heydel, Loïc Briand |
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| Přispěvatelé: | Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Université Bourgogne Franche-Comté [COMUE] (UBFC), Heydel, Jean-Marie |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Chimie-Physique
lcsh:Medicine Gas Chromatography-Mass Spectrometry 03 medical and health sciences 0302 clinical medicine Animals Metabolomics Food and Nutrition Rats Wistar lcsh:Science Volatile metabolites 030304 developmental biology 0303 health sciences Chemical Physics Multidisciplinary Chemistry lcsh:R Esters Metabolism Ketones Publisher Correction Rats Nasal Mucosa Biochemistry Models Animal Odorants Alimentation et Nutrition ComputingMethodologies_DOCUMENTANDTEXTPROCESSING lcsh:Q [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.8112. ⟨10.1038/s41598-019-44134-1⟩ Scientific Reports 1 (9), 8112. (2019) Scientific Reports, Vol 9, Iss 1, Pp 1-1 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Odorant-metabolizing enzymes are critically involved in the clearance of odorant molecules from the environment of the nasal neuro-olfactory tissue to maintain the sensitivity of olfactory detection. Odorant metabolism may also generate metabolites in situ, the characterization and function of which in olfaction remain largely unknown. Here, we engineered and validated an ex vivo method to measure odorant metabolism in real-time. Glassware containing an explant of rat olfactory mucosa was continuously flushed with an odorant flow and was coupled to a proton transfer reaction-mass spectrometer for volatile compound analysis. Focusing on carboxylic esters and diketone odorants, we recorded the metabolic uptake of odorants by the mucosa, concomitantly with the release of volatile odorant metabolites in the headspace. These results significantly change the picture of real-time in situ odorant metabolism and represent a new step forward in the investigation of the function of odorant metabolites in the peripheral olfactory process. Our method allows the systematic identification of odorant metabolites using a validated animal model and permits the screening of olfactory endogenously produced chemosensory molecules. |
| Databáze: | OpenAIRE |
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