Liposome Modified with VIP-Lipopeptide as a New Drug Delivery System
| Autor: | Ryosuke Imai, Yusaku Yokoyama, Hidemasa Hikawa, Ichirou Tatsuno, Hideharu Suzuki, Yuki Koide, Yingpeng Li, Akira Takami, Katsuhide Terada, Nobuhiro Yagi, Kenji Yano, Sho Kawatobi, Toru Masaka |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Pharmacology
chemistry.chemical_classification Liposome Cell Survival Vasoactive intestinal peptide Pharmaceutical Science Lipopeptide Peptide chemistry.chemical_compound Pituitary adenylate cyclase-activating peptide Drug Delivery Systems Biochemistry chemistry Targeted drug delivery Cell Line Tumor Liposomes Drug delivery Humans Receptor hormones hormone substitutes and hormone antagonists Vasoactive Intestinal Peptide |
| Zdroj: | YAKUGAKU ZASSHI. 134:987-995 |
| ISSN: | 1347-5231 0031-6903 |
| DOI: | 10.1248/yakushi.14-00019 |
| Popis: | We have designed a novel lipid analog (lipopeptide) that mimics the structural features of modified phospholipids. This lipopeptide is easily synthesized using a peptide synthesizer and has been shown to be useful for the modification of liposomes, which are used as an active targeted drug delivery system (DDS). Vasoactive intestinal peptide (VIP) has high homology with pituitary adenylate cyclase activating peptide (PACAP). There are three major PACAP receptors: PAC1R, VPAC1R, and VPAC2R. PAC1R has affinity only for PACAP, whereas VPAC1R and VPAC2R have the same affinity for both VIP and PACAP. In the present study, we synthesized several lipopeptides conjugated with VIP through different linkers and found that liposomes modified with these lipopeptides (VIP-Lips) selectively recognized the PACAP/VIP receptors. The anti-cancer activity of these VIP-Lips was evaluated by encapsulation of an antitumor drug, doxorubicin (DOX), into the modified liposomes (VIP-Lips-DOX) against the human osteosarcoma cell line, Saos-2, which highly expresses the VIP receptor. cAMP production was then measured to determine how well the VIP-Lips were able to recognize VPAC2R. The results clearly indicate that the proposed lipopeptide methodology holds promise as a DDS for cancer therapy. |
| Databáze: | OpenAIRE |
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