In situ double-beam NADH laser fluorimetry: choice of a reference wavelength
| Autor: | J. Cornillault, G. Renault, J. P. Berthier, M. Muffat-Joly, J. J. Pocidalo, E. Raynal, B. Godard, M. Sinet |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
In situ
Optical fiber Materials science Physiology Analytical chemistry Fluorescence spectrometry Signal law.invention law Physiology (medical) Fluorometer Animals Fiber Optic Technology Fluorometry Hypoxia Optical Fibers Lasers Myocardium Models Cardiovascular Laser NAD Fluorescence Rats Perfusion Wavelength Blood Biochemistry Hematocrit Cardiology and Cardiovascular Medicine Mathematics |
| Zdroj: | The American journal of physiology. 246(4 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | A double-beam laser fluorimeter, using a single optical fiber to guide the lights, was constructed for in situ and on-line monitoring of NADH concentration [( NADH]) from normally blood-perfused living tissues. The device was tested on an isolated blood-perfused rat heart system to determine the most efficient reference wavelength for the compensation of the hemodynamic artifact induced by blood circulation in the tissues on the fluorescence measure; 586 nm was found to be an accurate reference wavelength, and a mathematical relationship was established that allowed the digital treatment of the measured fluorescence to give a signal (compensated fluorescence) that varied only with [NADH] in the volume of tissue investigated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|