Ganoderma Lucidum induces oxidative DNA damage and enhances the effect of 5-Fluorouracil in colorectal cancer in vitro and in vivo
| Autor: | Tomas Hucl, Natalie Galanova, Peter Macinga, Josef Horak, Andrea Cumova, Daniel Sliva, Ludmila Vodickova, Katarína Kozics, Sona Vodenkova, Alexandra Rejhova, Pavel Vodicka, Alena Opattova, Klara Kostovcikova, Karolina Turnovcova |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Antimetabolites Antineoplastic Reishi Combination therapy Colorectal cancer DNA damage Health Toxicology and Mutagenesis medicine.medical_treatment 010501 environmental sciences Adenocarcinoma 01 natural sciences 03 medical and health sciences Mice In vivo Cell Line Tumor Genetics medicine Cytotoxic T cell Animals Neoplasm Invasiveness Tumor Stem Cell Assay 0105 earth and related environmental sciences Chemotherapy Mice Inbred BALB C business.industry Plant Extracts Drug Synergism DNA Neoplasm medicine.disease Tumor Burden Oxidative Stress 030104 developmental biology Fluorouracil Cancer cell Cancer research Female Comet Assay Drug Screening Assays Antitumor business Colorectal Neoplasms Reactive Oxygen Species Cell Division medicine.drug DNA Damage |
| Zdroj: | Mutation research. Genetic toxicology and environmental mutagenesis. 845 |
| ISSN: | 1879-3592 |
| Popis: | The first-line chemotherapy of colorectal cancer (CRC), besides surgery, comprises administration of 5-Fluorouracil (5FU). Apart from cytotoxic effect on cancer cells, 5FU may also cause adverse side effects. Ganoderma Lucidum (GLC) is a mushroom used in Traditional Eastern Medicine. We propose that natural compounds, particularly GLC extracts, may sensitize cancer cells to conventional chemotherapeutics. This combination therapy could lead to more selective cancer cell death and may improve the response to the therapy and diminish the adverse effects of anticancer drugs. Here we demonstrate that GLC induced oxidative DNA damage selectively in colorectal cancer cell lines, whereas it protected non-malignant cells from the accumulation of reactive oxygen species. Accumulation of DNA damage caused sensitization of cancer cells to 5FU resulting in improved anticancer effect of 5FU. The results obtained in colorectal cell lines were confirmed in in vivo study: GLC co-treatment with 5FU increased the survival of treated mice and reduced the tumor volume in comparison with group treated with 5FU alone. Combination of conventional chemotherapeutics and natural compounds is a promising approach, which may reduce the effective curative dose of anticancer drugs, suppress their adverse effects and ultimately lead to better quality of life of CRC patients. |
| Databáze: | OpenAIRE |
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