| Autor: |
Anna Roig, Antonio Artigas, Raquel Guillamat Prats, Lluis Blanch, Aina Areny Balagueró, Marta Camprubí Rimblas, Daniel Closa |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
TP52. TP052 ARDS STUDIES. |
| Popis: |
In this study we aimed to determine the effect of the exosomes derived from Mesenchymal stromal cells (MSC) on cell proliferation, and to study how pre-conditioning the MSCs to a septic environment, changes their exosomes’ content and consequently, their regenerative capacity. MSC were isolated from male rats’ femora and tibiae and cultured in non pre-stimulated and pre-stimulated (with LPS) conditions. The secreted exosomes were obtained via standard ultracentrifugation protocol. Cell proliferation experiments were conducted by treating an epithelial cell line with exosomes from non pre-stimulated MSC (Control exosomes) and pre-stimulated MSC (LPS exosomes) through an in vitro scratch assay. Exosomal protein profile was analyzed by a liquid chromatography-mass spectrometry analysis. The treatment of epithelial cells with LPS exosomes increases a 10% its capacity to proliferate and to heal the wound in comparison with the cells treated with Control exosomes (Fig.1a). The analysis of the protein content of both types of exosomes confirmed that pre-conditioning MSCs to a septic environment modifies the protein cargo of its exosomes (Fig. 1b), resulting in the appearance proteins related to cell cycle regulation and cell proliferation. In conclusion, pre-stimulating MSC with LPS enhances their paracrine activity and improves the reparative capacity of their secreted exosomes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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