Identification of new Trichoderma strains with antagonistic activity against Botrytis cinerea
Autor: | Anton Harbuzov, Lidia Sas Paszt, Aleksandra Bogumił, Paweł Trzciński, Anna Lisek |
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Jazyk: | angličtina |
Rok vydání: | 2013 |
Předmět: |
chemistry.chemical_classification
Siderophore trichoderma spp biology Biological pest control food and beverages Plant culture grey mould Horticulture biology.organism_classification Microbiology SB1-1110 Enzyme chemistry Trichoderma Chitinase biology.protein identification antagonistic potential Incubation Mycelium Botrytis cinerea |
Zdroj: | Folia Horticulturae, Vol 25, Iss 2, Pp 123-132 (2013) |
ISSN: | 2083-5965 |
Popis: | The antagonistic activity of 52 isolates of Trichoderma spp. against Botrytis cinerea was tested in in vitro conditions using the dual culture technique. The results revealed that all of the Trichoderma isolates had the ability to inhibit the mycelial growth of grey mould. The percentage reduction in the growth of Botrytis cinerea after six days of incubation at 25oC varied between 45-78%. The isolates Tr43 and Tr52 showed the highest antagonistic activity (Tr43 – 76%; Tr52 – 78%). Biochemical and molecular identification indicated that both isolates were T . atroviride . The isolates showed differences in the utilisation of 11 to 96 different carbon sources. Additional biochemical tests revealed the ability of Tr43 and Tr52 to produce siderophores, indole-3-acetic acid and chitinases. Neither of the isolates gave positive results regarding phosphate solubilisation on Pikovskaya’s medium.Key words: antagonistic potential, grey mould, identification, Trichoderma spp. Identification of new |
Databáze: | OpenAIRE |
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