Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removal

Autor: Jelena Krunic, Ljiljana Đukić, Jelena Roganović, Nikola Stojanovic, Branka Popovic, Ivana Simic, Dragica Lj. Stojić
Rok vydání: 2018
Předmět:
Adult
Male
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Adolescent
Disinfectant
Biocompatible Materials
Enzyme-Linked Immunosorbent Assay
Dental Caries
Real-Time Polymerase Chain Reaction
Gastroenterology
Superoxide dismutase
03 medical and health sciences
Ozone
0302 clinical medicine
stomatognathic system
Lactobacillus
Internal medicine
medicine
Dentin
Humans
General Dentistry
Dental Pulp
Permanent teeth
biology
Superoxide Dismutase
Chemistry
Chlorhexidine
030206 dentistry
biology.organism_classification
Anti-Bacterial Agents
3. Good health
stomatognathic diseases
medicine.anatomical_structure
Antibacterial effect
030220 oncology & carcinogenesis
biology.protein
Pulp (tooth)
Biocompatibility
Female
Incomplete caries removal
Nitric Oxide Synthase
Residual caries
Caries Removal
medicine.drug
Zdroj: Clinical Oral Investigations
ISSN: 1436-3771
1432-6981
DOI: 10.1007/s00784-018-2495-x
Popis: ObjectivesTo evaluate local effect of gaseous ozone on bacteria in deep carious lesions after incomplete caries removal, using chlorhexidine as control, and to investigate its effect on pulp vascular endothelial growth factor (VEGF), neuronal nitric oxide synthase (nNOS), and superoxide dismutase (SOD).Materials and methodsAntibacterial effect was evaluated in 48 teeth with diagnosed deep carious lesion. After incomplete caries removal, teeth were randomly allocated into two groups regarding the cavity disinfectant used: ozone (open system) or 2% chlorhexidine. Dentin samples were analyzed for the presence of total bacteria and Lactobacillus spp. by real-time quantitative polymerase chain reaction. For evaluation of ozone effect on dental pulp, 38 intact permanent teeth indicated for pulp removal/tooth extraction were included. After cavity preparation, teeth were randomly allocated into two groups: ozone group and control group. VEGF/nNOS level and SOD activity in dental pulp were determined by enzyme-linked immunosorbent assay and spectrophotometric method, respectively.ResultsOzone application decreased number of total bacteria (p=0.001) and Lactobacillus spp. (p lt 0.001), similarly to chlorhexidine. The VEGF (p lt 0.001) and nNOS (p=0.012) levels in dental pulp after ozone application were higher, while SOD activity was lower (p=0.001) comparing to those in control pulp.ConclusionsAntibacterial effect of ozone on residual bacteria after incomplete caries removal was similar to that of 2% chlorhexidine. Effect of ozone on pulp VEGF, nNOS, and SOD indicated its biocompatibility.Clinical relevanceOzone appears as effective and biocompatible cavity disinfectant in treatment of deep carious lesions by incomplete caries removal technique.
Databáze: OpenAIRE
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