Genetic amplification of the transcriptional response to hypoxia as a novel means of identifying regulators of angiogenesis
| Autor: | Robert Alan Harris, Toby Price, Sheena R Lee, Marie H Craigon, Katie Binley, Christopher Robert Mundy, Stuart Naylor, Georgina L Beard, Jonathan White |
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| Rok vydání: | 2004 |
| Předmět: |
Transcription
Genetic Angiogenesis Genetic Vectors Neovascularization Physiologic Biology Adenoviridae Viral vector Neovascularization Transcriptome Genes Regulator Basic Helix-Loop-Helix Transcription Factors Genetics medicine Humans Transcription factor Cells Cultured Regulation of gene expression Reverse Transcriptase Polymerase Chain Reaction EPAS1 Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit Molecular biology Cell Hypoxia Cell biology Gene Expression Regulation Trans-Activators RNA medicine.symptom Transcription Factors |
| Zdroj: | Genomics. 83:1-8 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/s0888-7543(03)00215-5 |
| Popis: | The cellular response to hypoxia involves the promotion of angiogenesis, leading to increased blood flow and oxygenation. The macrophage has been identified as an orchestrator of this response in several pathologies, through the release of angiogenic factors in response to hypoxia. We have produced the first comprehensive transcriptome analysis of hypoxic primary human macrophages with respect to the regulation of angiogenesis. There is a marked induction of genes encoding factors known to stimulate angiogenesis, rather than factors that inhibit this process. We show that overexpression of the transcription factor EPAS1 using a recombinant adenoviral vector amplifies the induction of genes encoding angiogenic proteins in response to hypoxia. This defines a new strategy for enhancing transcriptome and proteome analyses by overexpressing disease-implicated genes using viral gene transfer methodologies. |
| Databáze: | OpenAIRE |
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