Intermediate monocytes exhibit higher levels of TLR2, TLR4 and CD64 early after congenital heart surgery
| Autor: | Nicole Ritz, Berthold Klein, Tobias Rubner, Tsvetomir Loukanov, Moritz B. Merbecks, Victoria C. Ziesenitz, Helmut Rauch, Patrick Saur, Noemi Rebecca Meier, Steffen Schmitt, Matthias Gorenflo |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Heart Defects Congenital Male medicine.medical_specialty Mean arterial pressure Heart disease Neutrophils Immunology Inflammation Biochemistry Monocytes law.invention 03 medical and health sciences 0302 clinical medicine law Cardiopulmonary bypass Immunology and Allergy Medicine Humans Arterial Pressure Prospective Studies Prospective cohort study Molecular Biology business.industry Organ dysfunction Receptors IgG Infant Newborn Infant Hematology medicine.disease Toll-Like Receptor 2 Surgery Toll-Like Receptor 4 030104 developmental biology Blood pressure Methylprednisolone 030220 oncology & carcinogenesis Cytokines Female medicine.symptom business medicine.drug |
| Zdroj: | Cytokine. 133 |
| ISSN: | 1096-0023 |
| Popis: | Introduction Congenital heart surgery with cardiopulmonary bypass (CPB) initiates an immune response which frequently leads to organ dysfunction and a systemic inflammatory response. Complications associated with exacerbated immune responses may severely impact the postoperative recovery. The objective was to describe the characteristics of monocyte subpopulations and neutrophils at the level of pattern recognition receptors (PRR) and the cytokine response after CPB in infants. Methods An observational cohort study was conducted between June 2016 and June 2017 of infants Results Samples from 21 infants (median age 7.4 months) were analyzed by flow cytometry and from 11 infants, cytokine concentrations were measured. Classical and intermediate monocytes showed first receptor upregulation with an increase in CD64 expression four hours post CPB. CD64–expression on intermediate monocytes almost tripled 48 h post CPB (p TLR4 expression on intermediate monocytes correlated with vasoactive-inotropic score (rs = 0.642, p = 0.0017), duration of ventilation (rs = 0.485, p = 0.0259), highest serum creatinine (rs = 0.547, p = 0.0102), postsurgical transfusion (total volume per kg bodyweight) (rs = 0.469, p = 0.0321) and lowest mean arterial pressure (rs = -0.530, p = 0.0135). Concentrations of IL-10, MIP-1β, IL-8, G–CSF and IL-6 increased one hour post CPB. Methylprednisolone administration in six patients had no significant influence on the studied surface receptors but led to lower IL-8 and higher IL-10 plasma concentrations. Conclusions Congenital heart surgery with CPB induces a systemic inflammatory process including cytokine response and changes in PRR expression. Intermediate monocytes feature specific inflammatory characteristics in the 48 h after pediatric CPB and TLR4 correlates with poorer clinical course, which might provide a potential diagnostic or even therapeutic target. |
| Databáze: | OpenAIRE |
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