Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
| Autor: | Martin Klabusay, Brandon J. Tefft, Dan Dragomir-Daescu, J. Jonathan Harburn, Gurpreet S. Sandhu, Susheil Uthamaraj |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Ferrofluid
General Immunology and Microbiology Chemistry Endosome General Chemical Engineering General Neuroscience Contrast Media Nanoparticle Bioengineering Nanotechnology Gene delivery Cell sorting General Biochemistry Genetics and Molecular Biology Paramagnetism PLGA chemistry.chemical_compound Drug Delivery Systems Polylactic Acid-Polyglycolic Acid Copolymer Animals Humans Lactic Acid Magnetite Nanoparticles Polyglycolic Acid Magnetite |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | Targeted delivery of cells and therapeutic agents would benefit a wide range of biomedical applications by concentrating the therapeutic effect at the target site while minimizing deleterious effects to off-target sites. Magnetic cell targeting is an efficient, safe, and straightforward delivery technique. Superparamagnetic iron oxide nanoparticles (SPION) are biodegradable, biocompatible, and can be endocytosed into cells to render them responsive to magnetic fields. The synthesis process involves creating magnetite (Fe3O4) nanoparticles followed by high-speed emulsification to form a poly(lactic-co-glycolic acid) (PLGA) coating. The PLGA-magnetite SPIONs are approximately 120 nm in diameter including the approximately 10 nm diameter magnetite core. When placed in culture medium, SPIONs are naturally endocytosed by cells and stored as small clusters within cytoplasmic endosomes. These particles impart sufficient magnetic mass to the cells to allow for targeting within magnetic fields. Numerous cell sorting and targeting applications are enabled by rendering various cell types responsive to magnetic fields. SPIONs have a variety of other biomedical applications as well including use as a medical imaging contrast agent, targeted drug or gene delivery, diagnostic assays, and generation of local hyperthermia for tumor therapy or tissue soldering. |
| Databáze: | OpenAIRE |
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