Role of Nucleolin in Endometrial Precancerous Hyperplasia and Carcinogenesis: Ex Vivo and In Silico Study
| Autor: | Vanya D. Barzilova, Josephine Drury, Bryony Rogers, Emily Thomas, Fareen Ahmed, Alice Bradfield, Hannan Al-Lamee, Dharani K. Hapangama |
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| Rok vydání: | 2022 |
| Předmět: |
Hyperplasia
Carcinogenesis Organic Chemistry RNA-Binding Proteins General Medicine Phosphoproteins Catalysis Endometrial Neoplasms endometrial cancer nucleolin metastasis Computer Science Applications Inorganic Chemistry Endometrium Carcinosarcoma Endometrial Hyperplasia Humans Female Physical and Theoretical Chemistry Precancerous Conditions Molecular Biology Spectroscopy |
| Zdroj: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 6228 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23116228 |
| Popis: | Endometrial cancer (EC) is the most common gynaecological malignancy. Nucleolin (NCL) is involved in rDNA transcription, cell proliferation, and apoptosis, with high expression associated with worse overall survival (OS) in other adenocarcinomas. Our aims were to assess NCL gene and protein expression and explore the differential expression of NCL-associated genes (NAGs) in endometrial carcinogenesis. Endometrial samples were obtained from 157 women to include healthy, hyperplastic (EH), EC, and metastatic groups. RT-qPCR and immunohistochemistry were employed to assess NCL gene and protein levels. In silico analysis of NAGs in TCGA and GEO datasets was performed, with the prognostic value determined via Human Protein Atlas. NCL mRNA level of EC was lower than in healthy post-menopausal endometrium (p < 0.01). EH samples had lower NCL immuno-expression scores than healthy pre-menopausal (p < 0.001), benign post-menopausal (p < 0.01), and EC (p < 0.0001) samples. Metastatic lesions demonstrated higher NCL quick scores than primary tissue (p = 0.04). Higher NCL Immuno quick scores carried a worse OS in high-grade EC (p = 0.01). Interrogating Uterine Corpus Endometrial Carcinoma (TCGA-UCEC) and Uterine Carcinosarcoma (TCGA-UCS) cohorts revealed NCL to be the most highly upregulated gene in carcinosarcoma, with S100A11, LMNB2, RERG, E2F1 and CCNA2 representing key dysregulated NAGs in EC. Since NCL is implicated in transforming hyperplastic glands into cancer, with further involvement in metastasis, it is suggested to be a promising target for better-informed diagnosis, risk stratification, and management of EC. |
| Databáze: | OpenAIRE |
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