In vitro production of porcine zygotes using intracytoplasmic injection of vitrified sperm
| Autor: | Gabriel Martín Alvarez, C. Baca Castex, Pablo Daniel Cetica, Claudia Cecilia Arraztoa, D. M. Neild |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Zygote medicine.medical_treatment Sus scrofa Embryonic Development Semen Fertilization in Vitro VITRIFICATION Cryopreservation Intracytoplasmic sperm injection Ciencias Biológicas Andrology Embryo Culture Techniques PORCINE 03 medical and health sciences PRONUCLEI 0302 clinical medicine Endocrinology Human fertilization medicine Animals Sperm Injections Intracytoplasmic 030219 obstetrics & reproductive medicine Pronucleus urogenital system Chemistry Embryogenesis 0402 animal and dairy science 04 agricultural and veterinary sciences INTRACYTOPLASMIC SPERM INJECTION 040201 dairy & animal science Sperm Spermatozoa Vitrification Oocytes Animal Science and Zoology Female Gases Biología Reproductiva CIENCIAS NATURALES Y EXACTAS SPERM Biotechnology |
| Zdroj: | Reproduction in domestic animals = Zuchthygiene. 52(5) |
| ISSN: | 1439-0531 |
| Popis: | The objectives of this study were to evaluate if vitrified porcine spermatozoa are able to maintain their capacity to produce zygotes in vitro using intracytoplasmic sperm injection (ICSI) and to evaluate the zygote development in two in vitro atmospheric conditions: 5% CO2 and tri-gas. A group of porcine oocytes maturated in vitro were injected with vitrified-warmed sperm (treatment group) and another group, with sperm diluted and conserved at 17°C (control group). To evidence parthenogenetic activation, some oocytes were submitted to a Sham test. The injected oocytes were cultured in G1 medium at 38°C, 100% humidity and 5% CO2 or tri-gas. No significant differences (p >.05) were observed in embryo development between the oocytes injected with vitrified-warmed sperm (31.8%; 36/113), and those injected with semen diluted and conserved at 17°C (35.5%; 32/90), when cultured in 5% CO2 or under tri-gas atmosphere (42.9%; 39/91 vs. 34.2%; 26/76, respectively). No significant differences (p >.05) were observed in the percentage of pronuclei (PN) obtained between 5% CO2 and tri-gas, within each treatment either. Of the 52 oocytes submitted to the Sham test, only two presented a female PN (activation) indicating that the PN observed in the treatment group were a product of fertilization and not parthenogenetic activation. To conclude, porcine sperm vitrified using spheres, at a concentration of 5 × 106 spermatozoa/ml in TALP medium with 1% bovine serum albumin (BSA), conserve condensed and intact chromatin capable of producing early embryo development up to the pronuclear stage. Fil: Arraztoa, Claudia Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alvarez, Gabriel Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina Fil: Neild, Debora Margarita. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina |
| Databáze: | OpenAIRE |
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