Polymer immobilized enzyme optrodes for the detection of penicillin
| Autor: | Stanley M. Angel, Irene. Camins, Christiane Munkholm, Thomas J. Kulp, David R. Walt |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
chemistry.chemical_classification
Chromatography Immobilized enzyme Polymers Polymer Penicillins Enzymes Immobilized Fluorescence Combinatorial chemistry Analytical Chemistry Penicillin chemistry.chemical_compound Membrane chemistry pH indicator medicine Optode Electrodes medicine.drug Penicilloic acid |
| Zdroj: | Analytical chemistry. 59(24) |
| ISSN: | 0003-2700 |
| Popis: | The preparation and performance of two enzyme-based fiber-optic sensors (optrodes) capable of detecting penicillin are described. Each sensor consists of a polymer membrane that is covalently attached to the tip of a glass optical fiber. The membrane contains the enzyme penicillinase and a pH-sensitive fluorescent dye. A signal is produced when the enzyme catalyzes the cleavage of the ..beta..-lactam ring of penicillin to produce penicilloic acid and, consequently, a pH change in the microenvironment of the membrane. The sensors differ in the way the polymer membrane is constructed and in the type of pH indicator dye used. Both optrodes exhibit response times (40-60 s) significantly lower than those of the corresponding enzyme electrodes (2 min). Each gives a linear response over the concentration range of 0.00025 to 0.01 M penicillin G, when measured in a 0.005 M phosphate buffer. The data indicate that these immobilization strategies produce similar results and may be considered complementary alternatives in future enzyme optrode applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|