In vitro selection of drug resistant Schistosoma mansoni
| Autor: | Gerald C. Coles, J.I. Bruce |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
Snails Drug Resistance Schistosomicides Drug resistance Pharmacology chemistry.chemical_compound Mice parasitic diseases Oltipraz medicine Animals biology Amoscanate Methane sulfonate Schistosoma mansoni biology.organism_classification Oxamniquine Praziquantel Infectious Diseases chemistry Mutation Parasitology Female medicine.drug |
| Zdroj: | International journal for parasitology. 17(3) |
| ISSN: | 0020-7519 |
| Popis: | Schistosomules of Schistosoma mansoni were cultured for 3 days in the presence of schistosomicides and then inoculated intraperitoneally into mice. Drug concentrations killing greater than 99.8% of schistosomules were amoscanate 0.1 p.p.m., oltipraz, 0.5 p.p.m., oxamniquine 240 p.p.m., praziquantel 8 p.p.m. Comparison of drug response of the unselected and selected strains as adult worms in mice showed an increase in tolerance to amoscanate, oltipraz and oxamniquine, but not praziquantel. The oxamniquine tolerant strain did not respond to oxamniquine at 500 mg kg −1 . The unselected strain increased in tolerance to three drugs during routine passage in the laboratory. Greater numbers of schistosomules derived from snails exposed to ethyl methane sulfonate appeared to survive culture in metrifonate, suggesting that it may be possible to produce drug resistant schistosomes by mutation and selection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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