A Simple Method to Generate Adipose Stem Cell-Derived Neurons for Screening Purposes
| Autor: | Claudia Verderio, Silvia Coco, Noemi Tonna, Caterina Bossio, Fabio Bianco, Raffaella Morini, Michela Matteoli, Rosa Mastrangelo |
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| Přispěvatelé: | Bossio, C, Mastrangelo, R, Morini, R, Tonna, N, Coco, S, Verderio, C, Matteoli, M, Bianco, F |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cellular differentiation
Neurogenesis Mesenchymal stem cells Neurons Differentiation Population Primary Cell Culture Adipose tissue Clinical uses of mesenchymal stem cells Biology Proteomics Rats Sprague-Dawley 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Neurosphere Adipocytes Animals education 030304 developmental biology Neurons 0303 health sciences education.field_of_study Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Anatomy Rats Calcium Female Stem cell Neuroscience 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Journal of Molecular Neuroscience Journal of Molecular Neuroscience; Vol 51 |
| ISSN: | 1559-1166 0895-8696 |
| DOI: | 10.1007/s12031-013-9985-8 |
| Popis: | Strategies involved in mesenchymal stem cell (MSC) differentiation toward neuronal cells for screening purposes are characterized by quality and quantity issues. Differentiated cells are often scarce with respect to starting undifferentiated population, and the differentiation process is usually quite long, with high risk of contamination and low yield efficiency. Here, we describe a novel simple method to induce direct differentiation of MSCs into neuronal cells, without neurosphere formation. Differentiated cells are characterized by clear morphological changes, expression of neuronal specific markers, showing functional response to depolarizing stimuli and electrophysiological properties similar to those of developing neurons. The method described here represents a valuable tool for future strategies aimed at personalized screening of therapeutic agents in vitro. |
| Databáze: | OpenAIRE |
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