Combined Omics Approaches Reveal the Roles of Non-canonical WNT7B Signaling and YY1 in the Proliferation of Human Pancreatic Progenitor Cells
| Autor: | Taro Toyoda, Azuma Kimura, Kenji Osafune, Ryusuke Hirama, Mio Iwasaki |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Small interfering RNA
WNT7B proliferation medicine.medical_treatment Clinical Biochemistry Cell Biology YY1 01 natural sciences Biochemistry Cell Line Transcriptome Mice pancreatic progenitor cells Drug Discovery medicine Transcriptional regulation Animals Humans Progenitor cell Induced pluripotent stem cell Pancreas Molecular Biology Protein Kinase C YY1 Transcription Factor Cell Proliferation Pharmacology non-canonical Wnt/PKC signaling pathway 010405 organic chemistry Growth factor Wnt signaling pathway Feeder Cells 0104 chemical sciences Cell biology Wnt Proteins medicine.anatomical_structure Molecular Medicine sense organs pluripotent stem cells Signal Transduction |
| Zdroj: | Cell Chemical Biology. 27:1561-1572.e7 |
| ISSN: | 2451-9456 |
| DOI: | 10.1016/j.chembiol.2020.08.018 |
| Popis: | The proliferation of human pancreatic progenitor cells (PPCs) is critical for developing cell therapies for diabetes. Here, using transcriptome analysis combined with small interfering RNA (siRNA) screening, we revealed that WNT7B is a downstream growth factor of AT7867, a compound known to promote the proliferation of PPCs generated from human pluripotent stem cells. Feeder cell lines stably expressing mouse Wnt7a or Wnt7b, but not other Wnts, enhanced PPC proliferation in the absence of AT7867. Importantly, Wnt7a/b ligands did not activate the canonical Wnt pathway, and PPC proliferation depended on the non-canonical Wnt/PKC pathway. A comparison of the phosphoproteome in response to AT7867 or a newly synthesized AT7867 derivative uncovered the function of YY1 as a transcriptional regulator of WNT7B. Overall, our data highlight unknown roles of non-canonical WNT7B/PKC signaling and YY1 in human PPC proliferation and will contribute to the stable supply of a cell source for pancreatic disease modeling and therapeutic applications. ヒト膵臓細胞の増殖メカニズムを解明 --糖尿病治療に向けて前進--. 京都大学プレスリリース. 2020-10-30. |
| Databáze: | OpenAIRE |
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