The metabolism of gastrin-52 and gastrin-6 in pigs
| Autor: | F. Stadil, Jens F. Rehfeld, Jens P. Goetze, C. Palnaes Hansen |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Swine Physiology Radioimmunoassay Biology Kidney Sulfation Physiology (medical) Internal medicine Gastrins medicine Animals Anesthesia Intestinal Mucosa Protein Precursors Gastrin Hepatology Sulfates Hemodynamics Gastroenterology Metabolism Hormones Peptide Fragments Kinetics Portal System Endocrinology Liver Gastrointestinal hormone Biochemistry Injections Intravenous Chromatography Gel |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 279:G552-G560 |
| ISSN: | 1522-1547 0193-1857 |
| Popis: | The kinetics and metabolism in various organs of three bioactive products of progastrin, the small sulfated and nonsulfated gastrin-6 and the large nonsulfated gastrin-52, were examined during intravenous administration in anesthetized pigs. The kidney, hindlimb, liver, head, and gut eliminated the hexapeptides efficiently, with a fractional extraction ranging from 0.50 to 0.28 ( P < 0.001–0.05). No metabolism was recorded in the lungs, and sulfation was without influence on the extraction of gastrin-6. Gastrin-52 was eliminated only in the kidney and the head, with a fractional extraction between 0.23 and 0.11 ( P < 0.01–0.05). The half-life of sulfated and nonsulfated gastrin-6 was 1.5 ± 0.4 and 1.4 ± 0.3 min, the metabolic clearance rate (MCR) was 80.8 ± 7.6 and 116.0 ± 13.5 ml · kg−1· min−1( P < 0.05), and the apparent volume of distribution (Vdss) was 199.3 ± 70.1 and 231.4 ± 37.3 ml/kg, respectively. The decay of gastrin-52 in plasma was biexponential. The half-lives of this biexponential after a bolus injection were 3.9 ± 0.5 ( T1/2α) and 25.7 ± 1.4 ( T1/2β) min, and the MCR and Vdsswere 4.2 ± 0.4 ml · kg−1· min−1and 116.2 ± 16.2 ml/kg1. We conclude that there is a differential elimination of progastrin products in splanchnic and nonsplanchnic tissue, which depends on the chain length of the peptides. Sulfation of gastrin-6 had no influence on the organ-specific extraction but reduced the MCR. Our results are in keeping with previous studies of nonsulfated gastrin-17, which is extracted in the kidney, head, limb, and gut but not in the liver. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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