Rapid three-dimensional biointerfaces for real-time immunoassay using hIL-18BPa as a model antigen
| Autor: | Shawn D. Carrigan, George Scott, Maryam Tabrizian |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Materials science
Surface Properties Transducers Biophysics Bioengineering Nanotechnology Biointerface Sensitivity and Specificity Biomaterials Antigen Coated Materials Biocompatible Computer Systems Materials Testing medicine Surface plasmon resonance Antigens Glycoproteins Immunoassay medicine.diagnostic_test Atomic force microscopy technology industry and agriculture Reproducibility of Results Quartz crystal microbalance Equipment Design Human serum albumin Equipment Failure Analysis Mechanics of Materials Self-healing hydrogels Ceramics and Composites Cytokines Intercellular Signaling Peptides and Proteins Biological Assay Biomedical engineering medicine.drug |
| Zdroj: | Biomaterials. 26(35) |
| ISSN: | 0142-9612 |
| Popis: | With the goal of designing a rapid and affordable system of real-time immune monitoring for future diagnostic applications in sepsis, we have developed a biointerface composed of polyethyleneimine (PEI) and carboxymethylcellulose (CMC) to provide a means of prompt and facile immunoassay. Biointerface assembly is complete within 30 min, with all preparation performed and monitored within the measurement chamber of a quartz crystal microgravimetry with dissipation (QCM-D) sensor. Optimised biointerface composition, as determined by the mass of antibody immobilised, the level of antigen detection, and the amount of non-specific binding of human serum albumin, was determined to consist of a 4.0 mg/m L CMC hydrogel layer cross-linked to a 0.5 mg/m L PEI sub-layer. Tapping mode atomic force microscopy (AFM) in liquid demonstrates highly uniform and smooth surfaces using these hydrogels. Sensitivity of the biointerface for rhIL-18BPa is 400 ng/m L , with detection of 1 μg/m L achievable following 25 surface regenerations. Performance of the biointerface is verified using surface plasmon resonance (SPR), demonstrating the ability of the biointerface to be applied across platforms. |
| Databáze: | OpenAIRE |
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