Accurate and Rapid Protein Concentration Measurement of In-Process, High Concentration Protein Pools
| Autor: | Sunitha Kandula, Nihal Tugcu, William S. McKechnie |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
High concentration Materials science Chromatography Absorption spectroscopy Measure (physics) Beer–Lambert law Proteins Reproducibility of Results 030226 pharmacology & pharmacy 01 natural sciences High-performance liquid chromatography 03 medical and health sciences symbols.namesake 0302 clinical medicine Ultraviolet visible spectroscopy Path length 010608 biotechnology symbols Spectrophotometry Ultraviolet Spectroscopy Chromatography High Pressure Liquid Biotechnology |
| Zdroj: | Biotechnology progress. 34(5) |
| ISSN: | 1520-6033 |
| Popis: | Protein concentration is a critical product quality attribute and required for any therapeutic protein. Many commercial and investigational new biologics are now formulated at high concentrations (>100 mg/ml) to achieve successful subcutaneous administration. Assaying protein concentration in high concentration formulations poses a challenge, as traditional absorption spectroscopy and UPLC/HPLC (ultra/high performance liquid chromatography) assays cannot accurately measure such high concentrations without further solution manipulation. However, recent advances in UV/vis technology have led to the creation of instruments that measure samples at relatively short ( |
| Databáze: | OpenAIRE |
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