Elevation of Autoantibody in Patients with Ischemic Stroke
| Autor: | Yoichi Yoshida, Seiichiro Mine, Yasuo Iwadate, Masaki Takiguchi, Jun Matsushima, Takaki Hiwasa, Toshio Machida, Eiichi Kobayashi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Review Article
Serology Brain Ischemia 03 medical and health sciences 0302 clinical medicine Immune system Antigen Medicine Humans Autoantibodies biology business.industry Cerebral infarction Autoantibody TIA medicine.disease cerebral infarction Stroke 030220 oncology & carcinogenesis Immunology biology.protein Protein microarray Biomarker (medicine) biomarker Surgery Neurology (clinical) Antibody atherosclerosis business 030217 neurology & neurosurgery autoantibody |
| Zdroj: | Neurologia medico-chirurgica |
| ISSN: | 1349-8029 |
| Popis: | Recent clinical research has revealed a significant correlation between atherosclerosis, one of the primary etiologies of ischemic stroke, and the immune system. Assuming that "disease-specific autoantibodies are produced in the sera of patients with ischemic stroke," we investigated multiple arteriosclerosis-related antibodies using the serological identification of antigens by recombinant cDNA expression cloning (SEREX), an established method for identifying antigenic proteins. We either screened a human aortic endothelial cell cDNA library or conducted protein array screening using the sera from patients with ischemic stroke, such as carotid artery stenosis or transient ischemic attack (TIA). Next, we measured serum antibody levels using amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) in patient/healthy donor (HD) cohorts and identified several antigens, the antibody levels of which were significantly higher in patients with ischemic stroke than in HDs. This review introduced the method of identifying antigens by the SEREX and protein microarray and summarized antigenic proteins. In particular, it focused on anti-replication protein A2 antibody and anti-programmed cell death 11 antibody, which are significantly related to atherosclerotic plaque and ischemic brain tissue, respectively, and proposed the mechanism of elevated autoantibody levels against them. Furthermore, this review suggests a possibility of clinical application as an atherosclerotic disease diagnostic marker for TIA or cerebral infarction. |
| Databáze: | OpenAIRE |
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