DEC-205 receptor-mediated long-circling nanoliposome as an antigen and Eucommia ulmoides polysaccharide delivery system enhances the immune response via facilitating dendritic cells maturation

Autor: Linzi Zhang, Xiaonong Yang, Qianqian Liu, Haibo Feng, Dongkun Chai, Jing Fan
Jazyk: angličtina
Rok vydání: 2020
Předmět:
nanoliposome
ved/biology.organism_classification_rank.species
Pharmaceutical Science
Receptors
Cell Surface
Eucommia ulmoides
02 engineering and technology
RM1-950
Polysaccharide
030226 pharmacology & pharmacy
immune response
Minor Histocompatibility Antigens
Mice
03 medical and health sciences
0302 clinical medicine
Immune system
Antigen
Antigens
CD
Polysaccharides
Animals
Immunologic Factors
Lectins
C-Type
dendritic cells
Antigens
Cells
Cultured
chemistry.chemical_classification
Mice
Inbred ICR
Chemistry
ved/biology
Eucommiaceae
Immunity
eucommia ulmoides polysaccharide
General Medicine
Receptor-mediated endocytosis
021001 nanoscience & nanotechnology
dec-205 receptor
Cell biology
Liposomes
Nanoparticles
Female
Delivery system
Therapeutics. Pharmacology
0210 nano-technology
Research Article
Zdroj: Drug Delivery, Vol 27, Iss 1, Pp 1581-1596 (2020)
Drug Delivery
article-version (VoR) Version of Record
ISSN: 1521-0464
1071-7544
Popis: DEC-205 receptor-mediated dendritic cells (DC) targeting nanoliposomes is a promising delivery system in eliciting an immune response against pathogens. When this delivery system carries both antigen and immunomodulator, it can effectively regulate the DC function as well as the initial T cell response. To maximize the desired therapeutic effects of Eucommia ulmoides Oliv. polysaccharides (EUPS), and induce an efficient humoral and cellular immune response against an antigen, we encapsulated the OVA and EUPS in long-circling nanoliposomes and conjugated it with anti-DEC-205 receptor antibody to obtain a DEC-205-targeted nanoliposomes (anti-DEC-205-EUPS-OVA-LPSM). The physicochemical properties and immune-modulating effects were investigated in vitro and in vivo by a series of the experiment to evaluate the targeting efficiency of anti-DEC-205-EUPS-OVA-LPSM. In vitro, anti-DEC-205-EUPS-OVA-LPSM (160 μg mL−1) could enhance DCs proliferation and increase their phagocytic efficiency. In vivo anti-DEC-205-EUPS-OVA-LPSM remarkably promoted the OVA-specific IgG and IgG isotypes levels, enhanced the splenocyte proliferation, and induced the NK cell and CTL cytotoxicity. Besides, the anti-DEC-205-EUPS-OVA-LPSM enhanced the maturation of DCs. These findings suggest that the DEC-205 receptor antibody-conjugated EUPS nanoliposome can act as an efficient antigen delivery system to enhance the cellular and humoral immune response by promoting DC maturation. This indicates that the anti-DEC-205-EUPS-OVA-LPSM has significant potential as an immune-enhancing agent and antigen delivery system.
Databáze: OpenAIRE
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