Osteopontin Overexpression Induced Tumor Progression and Chemoresistance to Oxaliplatin through Induction of Stem-Like Properties in Human Colorectal Cancer
Autor: | Thomas Yau, Lui Ng, Chi Chung Foo, Roberta Pang, Johnny Hon Wai Man, Jensen T. C. Poon, Oswens Siu-Hung Lo, Wai Lun Law, Ariel Chow, Guanghua Chen, Deepak Narayanan Iyer, Ronnie Tung-Ping Poon, Timothy Ming-Hun Wan |
---|---|
Rok vydání: | 2015 |
Předmět: |
lcsh:Internal medicine
Pathology medicine.medical_specialty Article Subject Colorectal cancer medicine.medical_treatment Stem cell marker stomatognathic system SOX2 medicine Osteopontin lcsh:RC31-1245 neoplasms Molecular Biology Chemotherapy biology business.industry Cell Biology medicine.disease digestive system diseases In vitro Oxaliplatin Tumor progression Cancer research biology.protein business Research Article medicine.drug |
Zdroj: | Stem Cells International Stem Cells International, Vol 2015 (2015) |
ISSN: | 1687-9678 1687-966X |
Popis: | © 2015 Lui Ng et al.Colorectal cancer (CRC) is one of the most common and fatal malignancies worldwide. The poor prognosis of colorectal cancer patients is due to development of chemoresistance and cancer metastasis. Recently osteopontin (OPN) has been associated with stem-like properties in colorectal cancer. This study further examined the clinicopathological significance of OPN in CRC and its effect on chemoresistance and transcription of stem cell markers. We examined the transcription level of OPN in 84 CRC patients and correlated the expression with their clinicopathological parameters. The associations of OPN overexpression with transcription of stem cell markers and response to chemotherapy in DLD1-OPN overexpressing clones and CRC patients were also investigated. Our results showed that OPN was significantly overexpressed in CRC, and its overexpression correlated with tumor stage and poor prognosis. Overexpression of CRC induced OCT4 and SOX2 expression in vitro and correlated with SOX2 overexpression in CRC patients. In addition, DLD1-OPN overexpressing cells showed enhanced ability to survive upon oxaliplatin treatment, and OPN expression was higher in CRC patients who were resistant to oxaliplatin-involved chemotherapy treatment. Thus, CRC cells overexpressing OPN demonstrated stem-like properties and OPN inhibition is a potential therapeutic approach to combat CRC progression and chemoresistance. published_or_final_version |
Databáze: | OpenAIRE |
Externí odkaz: |