Cytotoxic, Antitumor and Toxicological Profile of Passiflora alata Leaf Extract
| Autor: | Eliana B. Souto, Jorge Mauricio David, Sara Albuquerque dos Santos, Ricardo Luiz Cavalcanti de Albuquerque Júnior, Geraldo Célio Brandão, Luciana Nalone Andrade, Patrícia Severino, Ricardo Guimarães Amaral, Adriana Andrade Carvalho, Silvana Vieira Floresta Gomes, Sandra L Santos |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
natural products
Pharmaceutical Science Analytical Chemistry Mice 0302 clinical medicine Neoplasms Drug Discovery Peru Passiflora alata 0303 health sciences biology Traditional medicine Passiflora food and beverages 3. Good health medicine.anatomical_structure Chemistry (miscellaneous) 030220 oncology & carcinogenesis Molecular Medicine Heterografts cytotoxicity Brazil White pulp Spleen Antineoplastic Agents Article lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry In vivo Cell Line Tumor medicine Potency Animals Humans cancer MTT assay antitumor activity Physical and Theoretical Chemistry IC50 030304 developmental biology Cell Proliferation Flavonoids Science & Technology Cell growth Plant Extracts Organic Chemistry biology.organism_classification Plant Leaves |
| Zdroj: | Molecules, Vol 25, Iss 4814, p 4814 (2020) Molecules Volume 25 Issue 20 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1420-3049 |
| Popis: | Passiflora alata or passion fruit is a native flowering plant from Amazon, geographically spread from Peru to Brazil. The plant has long been used in folks medicine for its pharmacological properties and is included in the Brazilian Pharmacopoeia since 1929. The aim of this study was to evaluate the potential cytotoxic and antitumor activities of Passiflora alata leaf extract (PaLE) in S180-tumor bearing mice. The percentage of cell proliferation inhibition (% CPI) and IC50 in relation to 4 tumor cell lines were determined in PC3, K-562, HepG2 and S180 cell lines using the MTT assay. PaLE showed a CPI > 75% and greater potency (IC50 < 30 µ g/mL) against PC3 and S180 cell lines. PaLE showed antitumor activity in treatments intraperitoneally (36.75% and 44.99% at doses of 100 and 150 mg/kg/day, respectively). Toxicological changes were shown in the reduced body mass associated with reduced food consumption, increased spleen mass associated with histopathological increase in the white pulp of the spleen and increased number of total leukocytes with changes in the percentage relationship between lymphocytes and neutrophils. Our outcomes corroborate the conclusion that PaLE has antitumor activity in vitro and in vivo with low toxicity. |
| Databáze: | OpenAIRE |
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