Recombinant human bone morphogenetic protein induces bone formation
| Autor: | K M Kerns, T Harada, Paul Cordes, Vicki Rosen, Elizabeth A. Wang, Josephine S. D'alessandro, Rodney M. Hewick, David I. Israel, P LaPan, M Bauduy |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Multidisciplinary
Bone Development biology Bone morphogenetic protein 8A Bone morphogenetic protein 10 Proteins Bone morphogenetic protein 3 Bone morphogenetic protein Molecular biology Bone and Bones Recombinant Proteins Cell Line Rats Bone morphogenetic protein 7 Molecular Weight Bone morphogenetic protein 6 Bone morphogenetic protein 5 Cartilage GDF6 Bone Morphogenetic Proteins biology.protein Animals Humans Growth Substances Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 87(6) |
| ISSN: | 0027-8424 |
| Popis: | We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|