Identification of a Novel Marker for Primordial Smooth Muscle and Its Differential Expression Pattern in Contractile vs Noncontractile Cells
| Autor: | Donald F. Hunt, Rocco Falchetto, Lisa M. Dahm, Jill E. Hungerford, Chauncey W. Bowers, James P. Hoeffler, Jeffrey Shabanowitz, Charles D. Little |
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| Rok vydání: | 1997 |
| Předmět: |
Vascular smooth muscle
Cellular differentiation Muscle Proteins Coturnix Actinin Biology Muscle Smooth Vascular Article Epitope 03 medical and health sciences 0302 clinical medicine Antigen Animals Amino Acid Sequence Fluorescent Antibody Technique Indirect Cytoskeleton Cells Cultured Actin 030304 developmental biology 0303 health sciences Antibodies Monoclonal Cell Differentiation Cell Biology Actin cytoskeleton Molecular biology Actins Molecular Weight Actin Cytoskeleton Cytoskeletal Proteins Peptides Chickens Biomarkers 030217 neurology & neurosurgery Muscle Contraction |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.137.4.925 |
| Popis: | The assembly of the vessel wall from its cellular and extracellular matrix components is an essential event in embryogenesis. Recently, we used the descending aorta of the embryonic quail to define the morphological events that initiate the formation of a multilayered vessel wall from a nascent endothelial cell tube (Hungerford, J.E., G.K. Owens, W.S. Argraves, and C.D. Little. 1996. Dev. Biol. 178:375–392). We generated an mAb, 1E12, that specifically labels smooth muscle cells from the early stages of development to adulthood. The goal of our present study was to characterize further the 1E12 antigen using both cytological and biochemical methods. The 1E12 antigen colocalizes with the actin cytoskeleton in smooth muscle cells grown on planar substrates in vitro; in contrast, embryonic vascular smooth muscle cells in situ contain 1E12 antigen that is distributed in threadlike filaments and in cytoplasmic rosette-like patterns. Initial biochemical analysis shows that the 1E12 mAb recognizes a protein, M r = 100,000, in lysates of adult avian gizzard. An additional polypeptide band, M r = 40,000, is also recognized in preparations of lysate, when stronger extraction conditions are used. We have identified the 100-kD polypeptide as smooth muscle α-actinin by tandem mass spectroscopy analysis. The 1E12 antibody is an IgM isotype. To prepare a more convenient 1E12 immunoreagent, we constructed a single chain antibody (sFv) using recombinant protein technology. The sFv recognizes a single 100-kD protein in gizzard lysates. Additionally, the recombinant antibody recognizes purified smooth muscle α-actinin. Our results suggest that the 1E12 antigen is a member of the α-actinin family of cytoskeletal proteins; furthermore, the onset of its expression defines a primordial cell restricted to the smooth muscle lineage. |
| Databáze: | OpenAIRE |
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